Choi Mijoo, Lee Jae-Hoon, Lee Yun-Jung, Paik Hyun-Dong, Park Eunju
Department of Food and Nutrition, Kyungnam University, Changwon 51767, Korea.
Research Group of Food Processing, Korea Food Research Institute, Wanju 55365, Korea.
Foods. 2022 Jan 4;11(1):121. doi: 10.3390/foods11010121.
The objective of this study was to determine the immunomodulatory effects of egg yolk protein-water extract (EYW) on splenocyte proliferation, cytokine secretion, immunoglobulin production, and NK cell cytotoxic activity in BALB/c mice. The forced swimming test (FST) was used to provide a model for suppressing immune regulation. The proliferation of B cells in the EYW supplementation group was significantly increased from the level to which it was reduced by the FST (from 40.9% to 81.8%, < 0.05). EYW supplementation affected cytokine secretion of splenocytes. Levels of interleukin (IL)-2 and IL-10-as Th1 and Th2 cytokines, respectively-were decreased after the FST. However, EYW supplementation showed that secretion levels of these cytokines were significantly increased to pre-FST levels ( < 0.05). The production of immunoglobulins (IgA and IgG) was increased abnormally after the FST, whereas EYW supplementation significantly decreased it to pre-FST levels ( < 0.05). EYW supplementation also improved NK cell cytotoxic activity against YAC-1 tumor cells compared to the PC group ( < 0.05). These data suggest that EYW has potential as an immunomodulatory agent in the food and/or pharmaceutical industries.
本研究的目的是确定蛋黄蛋白水提取物（EYW）对BALB/c小鼠脾细胞增殖、细胞因子分泌、免疫球蛋白产生及自然杀伤（NK）细胞细胞毒性活性的免疫调节作用。强迫游泳试验（FST）用于提供一种抑制免疫调节的模型。EYW补充组中B细胞的增殖从FST使其降低的水平显著增加（从40.9%增至81.8%，P<0.05）。EYW补充影响脾细胞的细胞因子分泌。FST后，作为Th1和Th2细胞因子的白细胞介素（IL）-2和IL-10水平降低。然而，EYW补充表明这些细胞因子的分泌水平显著增加至FST前水平（P<0.05）。FST后免疫球蛋白（IgA和IgG）的产生异常增加，而EYW补充显著将其降低至FST前水平（P<0.05）。与阳性对照（PC）组相比，EYW补充还改善了NK细胞对YAC-1肿瘤细胞的细胞毒性活性（P<0.05）。这些数据表明，EYW在食品和/或制药行业具有作为免疫调节剂的潜力。
