Department of Obstetrics and Gynecology, Keimyung University School of Medicine, Daegu 42601, Korea.
Department of Immunology, Keimyung University School of Medicine, Daegu 42601, Korea.
Int J Environ Res Public Health. 2021 Dec 23;19(1):107. doi: 10.3390/ijerph19010107.
We investigated whether cervical spp. colonization affects the intensity of inflammatory mediators in amniotic fluid retrieved during cesarean delivery in singleton preterm birth. One hundred fifty-three cases in singleton preterm birth with 24-34 weeks' gestation were enrolled. The intensities of seven inflammatory mediators (interleukin (IL)-1β, IL-6, IL-8, IL-10, tumor necrosis factor-α, and matrix metalloproteins (MMP)-8, MMP-9) of amniotic fluid were measured. We tested cervical swab specimens using real-time polymerase chain reaction assays to detect spp. colonization. Histologic chorioamnionitis (HCA) was diagnosed when acute inflammation was observed in any of the placental tissues. Mean gestational age at delivery and birth weight were 30.9 ± 2.4 weeks and 1567 ± 524 g, respectively. Cervical spp. colonization was detected 78 cases. The incidence of HCA was 32.3% (43/133). Although the intensities of all inflammatory mediators were significantly different according to presence or absence of HCA, there were no significant differences according to cervical spp. colonization. In all 43 cases with HCA and 90 cases without HCA, there were no significant differences between cervical spp. colonization and the intensity of inflammatory mediators. Cervical spp. colonization did not affect the intensity of inflammatory mediators in the amniotic fluid retrieved during cesarean delivery.
我们研究了宫颈 spp. 定植是否会影响剖宫产术中获取的羊水炎症介质的强度，这些孕妇均为单胎早产且妊娠 24-34 周。共纳入 153 例单胎早产 24-34 周孕妇，测量羊水七种炎症介质（白细胞介素（IL）-1β、IL-6、IL-8、IL-10、肿瘤坏死因子-α以及基质金属蛋白酶（MMP）-8、MMP-9）的强度。我们使用实时聚合酶链反应检测宫颈拭子标本以检测 spp. 定植。当胎盘组织有任何急性炎症时，诊断为组织学绒毛膜羊膜炎（HCA）。分娩时的平均胎龄和出生体重分别为 30.9±2.4 周和 1567±524g。检测到 78 例宫颈 spp. 定植。HCA 的发生率为 32.3%（43/133）。尽管所有炎症介质的强度根据是否存在 HCA 有显著差异，但根据宫颈 spp. 定植情况没有显著差异。在所有 43 例 HCA 患者和 90 例无 HCA 患者中，宫颈 spp. 定植与炎症介质的强度之间均无显著差异。宫颈 spp. 定植不会影响剖宫产术中获取的羊水炎症介质的强度。
