Liu Jun, Liu Dunhua, Wu Xun, Pan Cuili, Wang Shuzhe, Ma Lu
School of Agriculture, Ningxia University, Yinchuan 750021, China.
School of Food & Wine, Ningxia University, Yinchuan 750021, China.
Animals (Basel). 2021 Dec 28;12(1):52. doi: 10.3390/ani12010052.
Abnormal iron metabolism can cause oxidative stress in broilers, and transport stress (TS) may potentially influence iron metabolism. However, the mechanisms by which TS affects iron metabolism are unclear. This study used quantitative proteome analysis based on tandem mass tag (TMT) to investigate the effects of TS on liver iron metabolism in broilers. Broilers (n = 24) reared under the same conditions were selected randomly into the transported group for 4 h (T2) and non-transported group (T1). Results showed that the serum iron level and total iron-binding capacity of broilers in the T2 were significantly higher than those in the T1 ( < 0.05). The liver iron content of broilers in the T2 (0.498 ± 0.058 mg·gprot) was significantly higher than that in the T1 (0.357 ± 0.035 mg·gprot), and the iron-stained sections showed that TS caused the enrichment of iron in the liver. We identified 1139 differentially expressed proteins (DEPs). Twelve DEPs associated with iron metabolism were identified, of which eight were up-regulated, and four were down-regulated in T2 compared with T1. Prediction of the protein interaction network for DEPs showed that FTH1, IREB2, and HEPH play vital roles in this network. The results provide new insights into the effects of TS on broilers' liver iron metabolism.
铁代谢异常会导致肉鸡氧化应激，而运输应激（TS）可能会影响铁代谢。然而，TS影响铁代谢的机制尚不清楚。本研究采用基于串联质谱标签（TMT）的定量蛋白质组分析方法，研究TS对肉鸡肝脏铁代谢的影响。将在相同条件下饲养的肉鸡（n = 24）随机分为运输4小时的运输组（T2）和非运输组（T1）。结果表明，T2组肉鸡的血清铁水平和总铁结合能力显著高于T1组（<0.05）。T2组肉鸡的肝脏铁含量（0.498±0.058 mg·gprot）显著高于T1组（0.357±0.035 mg·gprot），铁染色切片显示TS导致肝脏中铁的富集。我们鉴定出1139个差异表达蛋白（DEP）。鉴定出12个与铁代谢相关的DEP，其中8个上调，4个在T2组中与T1组相比下调。对DEP的蛋白质相互作用网络预测表明，FTH1、IREB2和HEPH在该网络中起重要作用。这些结果为TS对肉鸡肝脏铁代谢的影响提供了新的见解。
