Department of Global Innovative Drug, Graduate School of Chung-Ang University, College of Pharmacy, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 151-756, Korea.
Molecules. 2021 Dec 21;27(1):9. doi: 10.3390/molecules27010009.
Diabetes is a chronic metabolic disease that is a constant problem. Previous studies have reported that Savi. extracts are effective in treating diabetes and its complications. Benincasae Exocarpium (BE) is a fruit peel of that has been reported to be used for the prevention and treatment of metabolic diseases such as hyperglycemia, obesity, and hyperlipidemia. However, there are not enough studies on the compounds and bioassays to support the efficacy of BE. The inhibitory activity of the BE extracts and fractions against advanced glycation end-products (AGE) formation and α-glucosidase activity was evaluated. These assays are relevant for the treatment of type 2 diabetes and its complications. Based on these results, compounds - were isolated through bioassay-guided isolation. In addition, we developed a high-performance liquid chromatography (HPLC) method that can simultaneously analyze these 11 compounds. Activity evaluation of the compounds was also conducted, and eight compounds exhibited significant activity. Among these, flavonoid compounds showed strong activity. A quantitative evaluation of eight bioactive compounds (, -) was conducted. In conclusion, this study demonstrated the potential of BE for prevention and treatment of type 2 diabetes and its complications.
糖尿病是一种慢性代谢疾病，一直是个问题。先前的研究报道，Savi. 提取物在治疗糖尿病及其并发症方面有效。Benincasae Exocarpium (BE) 是 的果皮，据报道可用于预防和治疗代谢疾病，如高血糖、肥胖症和高血脂症。然而，关于 BE 的化合物和生物测定法的研究还不够充分，无法支持其疗效。评估了 BE 提取物和馏分对晚期糖基化终产物 (AGE) 形成和 α-葡萄糖苷酶活性的抑制活性。这些测定法与治疗 2 型糖尿病及其并发症有关。基于这些结果，通过生物测定指导分离法分离出了化合物 -。此外，我们还开发了一种可同时分析这 11 种化合物的高效液相色谱 (HPLC) 方法。还对化合物的活性进行了评估，有 8 种化合物表现出显著的活性。其中，类黄酮化合物表现出很强的活性。对 8 种生物活性化合物（、-）进行了定量评价。总之，本研究表明 BE 具有预防和治疗 2 型糖尿病及其并发症的潜力。
