Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 660-701, Korea.
Chin Med. 2011 Jun 24;6:25. doi: 10.1186/1749-8546-6-25.
Since oxidative stress has been implicated in a neurodegenerative disease such as Alzheimer's disease (AD), natural antioxidants are promising candidates of chemopreventive agents. This study examines antioxidant and neuronal cell protective effects of various fractions of the methanolic extract of Erigeron annuus leaf and identifies active compounds of the extract.
Antioxidant activities of the fractions from Erigeron annuus leaf were examined with [2,2-azino-bis(3-ethylbenz thiazoline-6-sulfonic acid diammonium salt)] (ABTS) and ferric reducing antioxidant power (FRAP) assays. Neuroprotective effect of caffeic acid under oxidative stress induced by H2O2 was investigated with [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) and lactate dehydrogenase (LDH) assays.
This study demonstrated that butanol fraction had the highest antioxidant activity among all solvent fractions from methanolic extract E. annuus leaf. Butanol fraction had the highest total phenolic contents (396.49 mg of GAE/g). Caffeic acid, an isolated active compound from butanol fraction, showed dose-dependent in vitro antioxidant activity. Moreover, neuronal cell protection against oxidative stress induced cytotoxicity was also demonstrated.
Erigeron annuus leaf extracts containing caffeic acid as an active compound have antioxidative and neuroprotective effects on neuronal cells.
由于氧化应激与阿尔茨海默病（AD）等神经退行性疾病有关，天然抗氧化剂是有前途的化学预防剂候选物。本研究考察了苍耳叶甲醇提取物的各种馏分的抗氧化和神经元细胞保护作用，并鉴定了提取物的活性化合物。
用[2,2-偶氮-双（3-乙基苯并噻唑啉-6-磺酸二铵盐）]（ABTS）和铁还原抗氧化能力（FRAP）测定法测定苍耳叶馏分的抗氧化活性。用[3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴盐]（MTT）和乳酸脱氢酶（LDH）测定法研究咖啡酸在 H2O2 诱导的氧化应激下的神经保护作用。
本研究表明，在苍耳叶甲醇提取物的所有溶剂馏分中，丁醇馏分的抗氧化活性最高。丁醇馏分总酚含量最高（396.49mg GAE/g）。从丁醇馏分中分离出的活性化合物咖啡酸表现出体外抗氧化活性的剂量依赖性。此外，还证明了对神经元细胞的氧化应激诱导的细胞毒性具有神经保护作用。
含有咖啡酸作为活性化合物的苍耳叶提取物具有抗氧化和神经保护作用，可保护神经元细胞。
