Abdel-Salam Nabil A, Ghazy Nabila M, Sallam Shaimaa M, Radwan Mohamed M, Wanas Amira S, ElSohly Mahmoud A, El-Demellawy Maha A, Abdel-Rahman Nevin M, Piacente Sonia, Shenouda Mary L
a Faculty of Pharmacy, Department of Pharmacognosy , Alexandria University , Alexandria , Egypt.
b National Center for Natural Products Research , University of Mississippi , Mississippi , MS , USA.
Nat Prod Res. 2018 Mar;32(6):702-706. doi: 10.1080/14786419.2017.1332602. Epub 2017 Jun 5.
Alcea rosea L. is widely cultivated in gardens of Egypt as an ornamental plant and it has a great history of folkloric medicinal uses. In the present work, phytochemical investigation of the alcoholic extract of the flowers of A. rosea L. led to the isolation of six flavonoids (1-6). Dihydrokaempferol-4'-O-β-d-glucopyranoside (1), dihydrokaempferol (2), kaempferol-3-O-[6″-(E-coumaroyl)]-β-d-glucopyranoside (3), kaempferol-3-O-β-d-glucopyranoside (4), Apigenin (5) and kaempferol-3-O-α-l-rhamnopyranosyl-(1'″→6″)-β-d-glucopyranoside (6). Four of the isolated compounds were evaluated for their antioxidant, immunostimulant and cytotoxic activities against HepG-2 cell line. Compound (3) showed potent cytotoxic activity against HepG-2 cell line with high selectivity towards hepatocellular carcinoma in vitro (with IC = 3.8 μg/mL). Compounds 1 and 2 exhibited significant antioxidant activity and compound 4 showed a significant immune stimulant activity. Compound 1 is isolated for the first time from genus Alcea and this is the first report for its biological investigation.
蜀葵在埃及的花园中作为观赏植物被广泛种植，并且有着悠久的民间药用历史。在本研究中，对蜀葵花醇提取物进行植物化学研究，从中分离出了六种黄酮类化合物（1 - 6）。二氢山奈酚 - 4'-O-β - D - 吡喃葡萄糖苷（1）、二氢山奈酚（2）、山奈酚 - 3 - O - [6″ - （E - 香豆酰基）] - β - D - 吡喃葡萄糖苷（3）、山奈酚 - 3 - O - β - D - 吡喃葡萄糖苷（4）、芹菜素（5）和山奈酚 - 3 - O - α - L - 鼠李吡喃糖基 - （1'″→6″） - β - D - 吡喃葡萄糖苷（6）。对分离得到的四种化合物针对HepG - 2细胞系的抗氧化、免疫刺激和细胞毒性活性进行了评估。化合物（3）对HepG - 2细胞系显示出较强的细胞毒性活性，在体外对肝癌细胞具有高选择性（IC = 3.8 μg/mL）。化合物1和2表现出显著的抗氧化活性，化合物4表现出显著的免疫刺激活性。化合物1首次从蜀葵属中分离得到，这也是其生物学研究的首次报道。
