KM Convergence Research Division, Korea Institution of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 34054, Republic of Korea.
Korean Convergence Medical Science Major, KIOM School, University of Science & Technology (UST), Daejeon 34054, Republic of Korea.
Nutrients. 2024 Sep 30;16(19):3323. doi: 10.3390/nu16193323.
BACKGROUND/OBJECTIVES: Isatidis Folium (IF) has been used in traditional medicine for various ailments, and recent research highlights its anti-inflammatory, antiviral, and detoxifying properties. This study investigated the anti-inflammatory effects of a hydroethanolic extract of IF (EIF) on inflammasomes and colitis.
Dextran sulfate sodium (DSS)-induced colitis model C57BL/6 mice were treated with DSS, mesalamine, or EIF (200 mg/kg). Parameters such as daily disease activity index (DAI), spleen weight, colon length, and histopathology were evaluated. Intestinal fibrosis, mucin, and tight junction proteins were assessed using Masson's trichrome, periodic acid-Schiff, and immunohistochemistry staining. RAW264.7 and J774a.1 macrophages were treated with EIF and lipopolysaccharide, with cell viability assessed via the cell counting kit-8 assay, nitric oxide (NO) production with Griess reagent, and cytokine levels with the enzyme-linked immunosorbent assay. NF-κB inhibition was analyzed using the luciferase assay, and phytochemical analysis was performed using UPLC-MS/MS.
EIF mitigated weight loss, reduced DAI scores, prevented colon shortening, and attenuated mucosal damage, fibrosis, and goblet cell loss while enhancing the tight junction protein occludin. The anti-inflammatory effects of EIF in RAW264.7 cells included reduced NO production, pro-inflammatory cytokines, and NF-κB activity, along with inhibition of NLRP3 inflammasome responses in J774a.1 cells. The key constituents identified were tryptanthrin, indigo, and indirubin.
Animal studies demonstrated the efficacy of EIF in alleviating colitis, suggesting its potential for treating inflammatory diseases.
背景/目的：青黛在传统医学中被用于治疗各种疾病，最近的研究强调了其抗炎、抗病毒和解毒的特性。本研究旨在探讨青黛水醇提取物（EIF）对炎症小体和结肠炎的抗炎作用。
采用葡聚糖硫酸钠（DSS）诱导的 C57BL/6 小鼠结肠炎模型，用 DSS、美沙拉嗪或 EIF（200mg/kg）进行治疗。评估每日疾病活动指数（DAI）、脾脏重量、结肠长度和组织病理学等参数。采用 Masson 三色染色、过碘酸希夫（PAS）染色和免疫组织化学染色评估肠道纤维化、黏蛋白和紧密连接蛋白。用 EIF 和脂多糖处理 RAW264.7 和 J774a.1 巨噬细胞，通过细胞计数试剂盒-8 法评估细胞活力，用 Griess 试剂评估一氧化氮（NO）的产生，用酶联免疫吸附试验评估细胞因子水平。用荧光素酶检测法分析 NF-κB 抑制情况，并用 UPLC-MS/MS 进行植物化学分析。
EIF 减轻了体重减轻，降低了 DAI 评分，防止了结肠缩短，并减轻了黏膜损伤、纤维化和杯状细胞丢失，同时增强了紧密连接蛋白 occludin。EIF 在 RAW264.7 细胞中的抗炎作用包括减少 NO 产生、促炎细胞因子和 NF-κB 活性，以及抑制 J774a.1 细胞中的 NLRP3 炎症小体反应。鉴定出的关键成分是色胺、靛蓝和靛玉红。
动物研究表明 EIF 缓解结肠炎的疗效，提示其在治疗炎症性疾病方面具有潜力。
