Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia.
College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia.
Molecules. 2022 Jan 4;27(1):298. doi: 10.3390/molecules27010298.
In this study, ultrasound-assisted extraction conditions were optimized to maximize the yields of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol from (aerial parts). The three UAE factors, extraction temperature (), extraction time (), and liquid to solid ratio (), were optimized using response surface methodology (RSM). A Box-Behnken design was used for experimental design and phytoconstituent analysis was performed using high-performance liquid chromatography-UV. The optimal extraction conditions were found to be a 64.2 °C extraction temperature, 52.1 min extraction time, and 25.2 mL/g liquid to solid ratio. The experimental values of sennoside A, sennoside B, aloe-emodin, emodin, and chrysophanol (2.237, 12.792, 2.457, 0.261, and 1.529%, respectively) agreed with those predicted (2.152, 12.031, 2.331, 0.214, and 1.411%, respectively) by RSM models, thus demonstrating the appropriateness of the model used and the accomplishment of RSM in optimizing the extraction conditions. Excellent antioxidant properties were exhibited by methanol extract obtained using the optimized extraction conditions with a DPPH assay (IC50 = 59.7 ± 1.93, µg/mL) and ABTS method (47.2 ± 1.40, µg/mL) compared to standard ascorbic acid.
在这项研究中，优化了超声辅助提取条件，以最大限度地提高（地上部分）中番泻苷 A、番泻苷 B、芦荟大黄素、大黄素和大黄酚的产量。使用响应面法（RSM）优化了三个 UAE 因素，即提取温度（）、提取时间（）和液固比（）。使用 Box-Behnken 设计进行实验设计，并用高效液相色谱-紫外法进行植物成分分析。发现最佳提取条件为 64.2°C 的提取温度、52.1 分钟的提取时间和 25.2 mL/g 的液固比。番泻苷 A、番泻苷 B、芦荟大黄素、大黄素和大黄酚的实验值（分别为 2.237%、12.792%、2.457%、0.261%和 1.529%）与 RSM 模型预测值（分别为 2.152%、12.031%、2.331%、0.214%和 1.411%）一致，表明所使用的模型是合适的，并且 RSM 成功地优化了提取条件。使用优化的提取条件获得的 甲醇提取物具有出色的抗氧化性能，DPPH 测定法（IC50=59.7±1.93，μg/mL）和 ABTS 法（47.2±1.40，μg/mL）的 IC50 值均优于标准抗坏血酸。
