Gerdes J, Pickartz H, Brotherton J, Hammerstein J, Weitzel H, Stein H
Institute of Pathology, Free University of Berlin, West Germany.
Am J Pathol. 1987 Dec;129(3):486-92.
The growth fraction (GF) and estrogen receptor (ER) status of 76 cases of breast cancer were investigated on frozen sections of the same tissue block by immunostaining with monoclonal antibodies (Ki-67 and anti ER antibody). In 55 cases of this series, the ER status was also determined by standard biochemical methods. Our study revealed an inverse correlation between GF and ER status. This negative relationship was most significant when both variables were determined immunohistologically in the same tissue block and less significant when GF was analyzed by immunohistology and ER status by biochemical methods. These data suggest that the immunohistologically assessed ER values characterize the receptor status of a given carcinoma better than the biochemical values, which leads to the conclusion that the immunohistologically determined close negative relationship between GF and ER reflects the actual in vivo situation of a given breast carcinoma case. However, the immunohistologic analysis also revealed that there is a proportion of exceptional cases, ie, those with a positive ER status and a large or moderately large GF. Because only half of the ER-positive breast carcinomas respond to endocrine therapy with objective remission., it is hypothesized that it might be preferentially these mentioned exceptional cases that fail to positively respond to endocrine treatment protocols.
采用单克隆抗体（Ki-67和抗雌激素受体抗体）免疫染色法，对76例乳腺癌同一组织块的冰冻切片进行生长分数（GF）和雌激素受体（ER）状态的研究。在该系列的55例病例中，ER状态也通过标准生化方法进行测定。我们的研究揭示了GF与ER状态之间呈负相关。当在同一组织块中通过免疫组织化学方法测定这两个变量时，这种负相关关系最为显著；而当通过免疫组织化学方法分析GF且通过生化方法分析ER状态时，这种负相关关系则不太显著。这些数据表明，与生化值相比，免疫组织化学评估的ER值能更好地表征特定癌的受体状态，由此得出结论，免疫组织化学测定的GF与ER之间紧密的负相关关系反映了特定乳腺癌病例的实际体内情况。然而，免疫组织化学分析还显示，存在一定比例的特殊病例，即那些ER状态为阳性且GF较大或中等的病例。由于只有一半的ER阳性乳腺癌对内分泌治疗有客观缓解反应，因此推测可能正是这些上述特殊病例对内分泌治疗方案没有积极反应。
