定义组成的纳米纤维基质维持人类诱导多能干细胞培养。
Nanofibrous Matrix of Defined Composition Sustains Human Induced Pluripotent Stem Cell Culture.
机构信息
Materials Science Institute of Seville (ICMS), Joint CSIC-University of Seville Center, C/Américo Vespucio 49, Isla de la Cartuja, Seville 41092, Spain.
Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain.
出版信息
ACS Appl Bio Mater. 2021 Apr 19;4(4):3035-3040. doi: 10.1021/acsabm.0c00425. Epub 2021 Apr 8.
Human induced pluripotent stem cells (hiPSCs) represent the most promising biological material for regenerative medicine applications. In this work, a 3D solid nanofibrous matrix of defined composition (Colamigel-S) consisting of 97 wt % gelatin, 2.6 wt % atelocollagen, and 0.4 wt % laminin has been reproducibly processed and characterized and exhibits a homogeneous nanofibrillar network of high surface area, interconnected microcavities, and typical D-periodic collagen fibril nanostructural features. The purpose of the study was to test the performance of Colamigel-S as substrate for in vitro hiPSCs culture, finding that these cells efficiently attach and grow keeping their characteristic stem morphology and undifferentiated state.
人诱导多能干细胞（hiPSCs）代表了再生医学应用中最有前途的生物材料。在这项工作中，一种具有明确组成的 3D 固体纳米纤维基质（Colamigel-S）已被可重复地加工和表征，其由 97wt%的明胶、2.6wt%的去端肽胶原和 0.4wt%的层粘连蛋白组成，具有均匀的纳米纤维网络、高表面积、相互连接的微腔和典型的 D 周期胶原纤维纳米结构特征。该研究的目的是测试 Colamigel-S 作为体外 hiPSCs 培养的基底的性能，结果发现这些细胞能够有效地附着和生长，保持其特征性的干细胞形态和未分化状态。
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