Sauvage Center for Molecular Sciences, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, P. R. China.
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, Guangdong Provincial Key Laboratory of Laser Life Science, and College of Biophotonics, South China Normal University, Guangzhou 510631, P. R. China.
ACS Appl Bio Mater. 2021 Feb 15;4(2):1760-1770. doi: 10.1021/acsabm.0c01509. Epub 2021 Jan 20.
Short-wavelength absorption and emission (<600 nm), hydrophobicity, and low selectivity have greatly restricted the biomedical applications of BODIPY. Herein, a series of mitochondria-targeted BODIPY nanoparticles with a cationic triphenylphosphine (TPP) group ( NPs) bearing different lengths of ethylene glycol (0-4 units), along with without a cationic TPP group for comparison, have been rationally designed and prepared to investigate the interplay between their structures and the related properties. Our studies found that NPs showed a tendency of aggregation and precipitation while NPs could be stable in aqueous solutions. Compared with , tailor-made possessed a high photothermal conversion efficiency (PCE) of 76.6 vs 9.0% and exhibited the highest photoinduced cytotoxicity. Upon NIR irradiation, the temperature induced by NPs increased rapidly from room temperature to 76.0 °C in vitro and 50.0 °C at the tumor site in vivo within 5 min. Furthermore, effective mitochondrial imaging in vitro, photothermal imaging (PTI), and photoacoustic imaging (PAI) in vivo were achieved. In this paper, we developed tailor-made photothermal agents for targeting mitochondria and enhancing the PTI and PAI performances, which could be potentially applied in clinical precision theranostics.
短波长吸收和发射（<600nm）、疏水性和低选择性极大地限制了 BODIPY 在生物医学中的应用。在此，设计并制备了一系列带有阳离子三苯基膦（TPP）基团（NPs）的线粒体靶向 BODIPY 纳米粒子，带有不同长度的乙二醇（0-4 个单元），以及不带阳离子 TPP 基团的 NPs 进行比较，以研究它们的结构与相关性质之间的相互作用。我们的研究发现，NPs 表现出聚集和沉淀的趋势，而 NPs 可以在水溶液中稳定存在。与 相比，定制的 具有 76.6%的高光热转换效率（PCE）和最高的光诱导细胞毒性。在近红外照射下，NPs 在体外从室温迅速升高至 76.0°C，在体内肿瘤部位在 5 分钟内升高至 50.0°C。此外，还实现了体外有效的线粒体成像、光热成像（PTI）和光声成像（PAI）。在本文中，我们开发了针对线粒体的定制光热试剂，以增强 PTI 和 PAI 性能，这可能在临床精准治疗中得到应用。
