Programa de Pós-Graduação em Ciência Animal, Escola de Ciências da Vida, Pontifícia Universidade Católica do Paraná - PUCPR, Curitiba, PR, 80215-901, Brazil.
Vet Dermatol. 2022 Apr;33(2):124-e36. doi: 10.1111/vde.13055. Epub 2022 Jan 11.
Previous studies have shown that patch testing with food extracts can assist formulation of elimination diets (ED) in human patients with suspected adverse food reactions (AFR). Little is known about the use of these tests in dogs.
To evaluate the effectiveness of a combination of prick and patch testing in current protocols, and food challenge (FC) tests in dogs with AFR.
Prick and patch tests were performed on 21 dogs with chronic, nonseasonal pruritus. Dogs then were fed an ED formulated on the basis of the results. All dogs with improved clinical signs then were challenged with a food to which there had been a positive reaction in the tests. Six dogs subsequently were challenged with a food to which they had been negative on testing. Pruritus Visual Analog Scale (pVAS) and Canine Atopic Dermatitis Extent and Severity Index, 4th iteration (CADESI-04) were evaluated on Day (D)0, D30 and D60 of the ED. Sensitivity (SE), specificity (SP), positive (PPV) and negative (NPV) predictive values, and the Kappa (κ)value were calculated.
Of the 21 dogs, there was a significant mean improvement in pVAS and CADESI-04 scores in 16 (76%) dogs after D30 (P < 0.01) and D60 (P < 0.01) of the ED. There were no statistical differences between D30 and D60. The combination of tests had SE, SP, PPV, NPV and κ values of 80%, 66.7%, 66.7%, 80% and -0.17, respectively.
The combination of prick and patch testing reached high values of SE and NPV. A diagnosis of AFR was made in 76% of the dogs, and test results were useful for the selection of an ED.
先前的研究表明，食物提取物斑贴试验可辅助拟定人类疑似食物不良反应患者的排除饮食（ED）。但人们对这些试验在犬中的应用知之甚少。
评估现行方案中组合式皮内试验和斑贴试验以及食物激发试验（FC）在食物过敏反应（AFR）犬中的有效性。
对 21 只患有慢性、非季节性瘙痒的犬进行皮内试验和斑贴试验。然后，根据试验结果为这些犬制定 ED。所有临床症状改善的犬均用试验中呈阳性反应的食物进行激发试验。随后，有 6 只犬用试验结果为阴性的食物进行激发试验。在 ED 的第 0、30 和 60 天，使用瘙痒视觉模拟量表（pVAS）和犬特应性皮炎严重程度指数第 4 次迭代版（CADESI-04）评估犬的瘙痒程度。计算敏感性（SE）、特异性（SP）、阳性预测值（PPV）、阴性预测值（NPV）和 Kappa（κ）值。
21 只犬中，有 16 只（76%）犬在 ED 的第 30 天（P＜0.01）和第 60 天（P＜0.01）后 pVAS 和 CADESI-04 评分有显著的平均改善。第 30 天和第 60 天之间无统计学差异。组合试验的 SE、SP、PPV、NPV 和 κ 值分别为 80%、66.7%、66.7%、80%和-0.17。
皮内试验和斑贴试验的组合具有较高的 SE 和 NPV 值。76%的犬被诊断为 AFR，试验结果有助于选择 ED。
