Fattah Bikhal, Arif Huner, Hamzah Haider
Department of Biology, College of Science, University of Sulaimani, Sulaymaniyah, Iraq.
Appl Biochem Biotechnol. 2022 May;194(5):2036-2046. doi: 10.1007/s12010-022-03805-y. Epub 2022 Jan 11.
Due to the presence of antibiotic-resistant genes, treatment options of clinical isolates are exceedingly limited. This study was aimed to fabricate, optimize, characterize, and evaluate the action of silver nanoparticles (AgNPs) against a clinical isolate of Enterococcus faecalis. A combination of cell-free supernatant (C-FS) of the filamentous fungus Fusarium solani and Gram-negative Comamonas aquatica for AgNP formation was proposed; the antigrowth and antibiofilm of AgNPs against E. faecalis harboring bla and bla genes were assessed. The ratio of 1:2 v/v (C-FS:AgNO) at pH 9.0 for 72 h in 1 mM AgNO were the optimal conditions for AgNP formation. UV-vis absorption peak appeared at 425 nm and the crystalline nature of synthesized particles was verified by X-ray diffraction (XRD). Fourier transform infrared spectroscopy (FTIR) analysis confirmed the interaction of protein molecules with the AgNPs. Transmission electron microscopy (TEM) analysis demonstrated that fabricated AgNPs were relatively monodispersed, approximately spherical, and of size 2-7.5 nm. bla and bla were detected in E. faecalis; the growth and biofilm of E. faecalis were significantly decreased by the action of 12.5 μg/mL AgNPs. This is the first study proposing alternative sources to form AgNPs via synergistic metabolites of F. solani and C. aquatica. The results here offer a foundation for developing an effective therapy using AgNPs against clinical pathogens.
由于存在抗生素抗性基因，临床分离株的治疗选择极为有限。本研究旨在制备、优化、表征和评估银纳米颗粒（AgNPs）对粪肠球菌临床分离株的作用。提出将丝状真菌茄病镰刀菌的无细胞上清液（C-FS）与革兰氏阴性水生丛毛单胞菌结合用于形成AgNP；评估了AgNPs对携带bla和bla基因的粪肠球菌的抗生长和抗生物膜作用。在1 mM硝酸银中，pH 9.0条件下1:2 v/v（C-FS:AgNO）比例反应72小时是形成AgNP的最佳条件。紫外可见吸收峰出现在425 nm处，通过X射线衍射（XRD）验证了合成颗粒的晶体性质。傅里叶变换红外光谱（FTIR）分析证实了蛋白质分子与AgNPs的相互作用。透射电子显微镜（TEM）分析表明，制备的AgNPs相对单分散，近似球形，尺寸为2-7.5 nm。在粪肠球菌中检测到bla和bla；12.5 μg/mL的AgNPs作用显著降低了粪肠球菌的生长和生物膜形成。这是第一项提出通过茄病镰刀菌和水生丛毛单胞菌的协同代谢产物形成AgNPs的替代来源的研究。这里的结果为开发使用AgNPs对抗临床病原体的有效疗法提供了基础。
