Ramasami Nishanthi, Dhayalan Manikandan, Selvaraj Malathi, Riyaz Savaas Umar Mohammed, Perumal Palani, Irudayaraj Santiagu Stephen, Rajagopal Rajakrishnan, Alfarhan Ahmed, Stalin Antony
Department of Biotechnology, College of Science and Humanities, SRMIST, Kattankulathur, Tamil Nadu 603203 India.
Department of Prosthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (Saveetha University), Chennai, TamilNadu 600 077 India.
Indian J Microbiol. 2024 Dec;64(4):1787-1804. doi: 10.1007/s12088-024-01234-5. Epub 2024 Mar 9.
The emergence of multidrug resistance in pathogenic organisms has caused growing concern, especially among healthcare providers, necessitating the development of new antimicrobial compounds. Resistance to metal nanoparticles is more challenging for any pathogen and thus paved a new avenue of research to formulate a new line of drugs combined with metal nanoparticles to treat microbial resistance. In this present investigation, green synthesised silver (AgNP), gold (AuNP), and platinum (PtNP) nanoparticles using the rind extract of the fruit of Garcinia mangostana L., were bioconjugated with Streptomycin. Visual colour change in solution was evidenced as the result of bioconjugation process and also significant shift in the UV-Vis spectra was recorded. The antibacterial activity against Streptomycin resistant was performed with bare and bioconjugates, AuNP and PtNP did not show any activity whereas their bioconjugates showed 100% activity and MIC was recorded as 0.1067ppm (SAuNP) and 34ppm (SPtNP), SAgNP and AgNP showed antibacterial activity but comparatively higher activity was exhibited by SAgNP. MIC for AgNP and SAgNP was recorded as 0.325 ppm and 0.187 ppm respectively. The results of cell viability test showed that the highest percentage of cell death was recorded with SAuNP treated cells (96%), followed by SPtNP (95.5%), SAgNP (95.4%) and AgNP (92.6%). The hemocompatibility was evaluated using human erythrocytes. No hemolysis was observed with any of the test compounds at their MIC. The results of SEM analysis supported the report as it showed the characteristic biconcave RBC cells with smooth surfaces, strongly suggesting hemocompatibility of the test compounds.
The online version contains supplementary material available at 10.1007/s12088-024-01234-5.
致病生物中多重耐药性的出现引起了越来越多的关注，尤其是在医疗保健提供者中，这使得开发新的抗菌化合物成为必要。对任何病原体来说，对金属纳米颗粒产生耐药性更具挑战性，因此开辟了一条新的研究途径，以配制结合金属纳米颗粒的新型药物来治疗微生物耐药性。在本研究中，使用山竹果皮提取物绿色合成的银（AgNP）、金（AuNP）和铂（PtNP）纳米颗粒与链霉素进行了生物共轭。溶液中的视觉颜色变化证明了生物共轭过程的结果，并且在紫外可见光谱中也记录到了显著的偏移。对链霉素耐药菌进行了抗菌活性测试，裸纳米颗粒和生物共轭物中，AuNP和PtNP没有显示任何活性，而它们的生物共轭物显示出100%的活性，最低抑菌浓度（MIC）记录为0.1067ppm（SAuNP）和34ppm（SPtNP），SAgNP和AgNP显示出抗菌活性，但SAgNP表现出相对较高的活性。AgNP和SAgNP的MIC分别记录为0.325ppm和0.187ppm。细胞活力测试结果表明，用SAuNP处理的细胞记录到最高的细胞死亡百分比（96%），其次是SPtNP（95.5%）、SAgNP（95.4%）和AgNP（92.6%）。使用人类红细胞评估了血液相容性。在测试化合物的MIC浓度下，未观察到任何溶血现象。扫描电子显微镜（SEM）分析结果支持了该报告，因为它显示了具有光滑表面的典型双凹红细胞，强烈表明测试化合物具有血液相容性。
在线版本包含可在10.1007/s12088-024-01234-5获取的补充材料。
