College of Horticulture, Gansu Agricultural University, Lanzhou, China.
PLoS One. 2022 Jan 11;17(1):e0262506. doi: 10.1371/journal.pone.0262506. eCollection 2022.
As the main forms of carbohydrates, starch and sucrose play a vital role in the balance and coordination of various carbohydrates. Lanzhou lily is the most popular edible lily in China, mainly distributed in the central region of Gansu. To clarify the relationship between carbohydrate metabolism and bulb development of Lanzhou lily, so as to provide a basis for the promotion of the growth and development in Lanzhou lily and its important economic value, we studied lily bulbs in the squaring stage, flowering stage, half withering stage and withering stage. The plant height, fresh weight of mother and daughter bulbs continued to increase during the whole growth period and fresh weight of stem and leaf began to decrease in the half withering stage. The content of starch, sucrose and total soluble sugar in the lily mother bulb accumulated mostly in the flowering, withering and half withering stages, respectively. Starch, sucrose and total soluble sugar accumulated in the daughter bulb with the highest concentration during the withering stage. In the transcription level, sucrose synthase (SuSy1) and sucrose invertase (INV2) expressed the highest in squaring stage, and the expression was significantly higher in the mother bulb than in the daughter bulb. In flowering stage, the expression levels of soluble starch synthase (SSS1), starch-branching enzyme (SBE) and adenosine diphosphate-glucose pyrophosphorylase (AGP1) genes were higher in the mother bulb than in the daughter bulb. Altogether, our results indicate that starch and sucrose are important for the bulb growth and development of Lanzhou lily.
作为碳水化合物的主要形式，淀粉和蔗糖在各种碳水化合物的平衡和协调中起着至关重要的作用。兰州百合是中国最受欢迎的食用百合，主要分布在甘肃省中部地区。为了阐明碳水化合物代谢与兰州百合鳞茎发育的关系，从而为促进兰州百合的生长发育及其重要的经济价值提供依据，我们对展叶期、初花期、半枯萎期和枯萎期的百合鳞茎进行了研究。整个生长期间，母鳞茎和子鳞茎的株高和鲜重持续增加，而茎和叶的鲜重在半枯萎期开始下降。百合母鳞茎中的淀粉、蔗糖和总可溶性糖含量在开花期、枯萎期和半枯萎期积累最多。在枯萎期，子鳞茎中淀粉、蔗糖和总可溶性糖的积累浓度最高。在转录水平上，蔗糖合酶（SuSy1）和蔗糖转化酶（INV2）在展叶期表达最高，且在母鳞茎中的表达显著高于子鳞茎。在开花期，母鳞茎中可溶性淀粉合酶（SSS1）、淀粉分支酶（SBE）和腺苷二磷酸葡萄糖焦磷酸化酶（AGP1）基因的表达水平高于子鳞茎。综上所述，我们的研究结果表明，淀粉和蔗糖对兰州百合鳞茎的生长发育具有重要意义。
