Tian Chang, Zhai Lisheng, Zhu Wenjing, Qi Xiangyu, Yu Zhongyu, Wang Haibin, Chen Fadi, Wang Likai, Chen Sumei
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Plants (Basel). 2022 Mar 30;11(7):936. doi: 10.3390/plants11070936.
Plant-specific TCP transcription factors play a key role in plant development and stress responses. shows higher cold tolerance than its ornamental polyploid counterpart. However, whether the gene family plays a role in conferring cold tolerance upon remains unknown. Here, we identified 23 genes in , systematically analyzed their phylogenetic relationships and synteny with TCPs from other species, and evaluated their expression profiles at low temperature. Phylogenetic analysis of the protein sequences suggested that CnTCP proteins fall into two classes and three clades, with a typical bHLH domain. However, differences between and Arabidopsis in predicted protein structure and binding sites suggested a unique function of CnTCPs in . Furthermore, expression profiles showed that expression of most were downregulated under cold conditions, suggesting their importance in plant responses to cold stress. Notably, expression of and of its predicted target genes, //, led to fast responses to cold. Overexpression of Arabidopsis led to hypersensitivity to cold, suggesting that might play a negative role in responses to cold stress. Our results provide a foundation for future functional genomic studies on this gene family in chrysanthemum.
植物特有的TCP转录因子在植物发育和应激反应中起关键作用。[具体植物名称]比其观赏多倍体对应物表现出更高的耐寒性。然而，[具体植物名称]的[基因家族名称]基因家族是否在赋予[具体植物名称]耐寒性方面发挥作用仍不清楚。在此，我们在[具体植物名称]中鉴定出23个[基因家族名称]基因，系统地分析了它们与其他物种TCPs的系统发育关系和共线性，并评估了它们在低温下的表达谱。蛋白质序列的系统发育分析表明，CnTCP蛋白分为两类和三个进化枝，具有典型的bHLH结构域。然而，[具体植物名称]与拟南芥在预测的蛋白质结构和结合位点上的差异表明CnTCPs在[具体植物名称]中具有独特的功能。此外，表达谱显示大多数[基因家族名称]基因在寒冷条件下表达下调，表明它们在植物对冷胁迫的反应中很重要。值得注意的是，[具体基因名称]及其预测的靶基因[靶基因名称]的表达导致对寒冷的快速反应。拟南芥[具体基因名称]的过表达导致对寒冷的超敏反应，表明[具体基因名称]可能在[具体植物名称]对冷胁迫的反应中起负作用。我们的结果为今后对菊花中该基因家族的功能基因组学研究奠定了基础。
