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评价两种活体蜱虫人工感染方法作为研究蜱传病毒与其蜱媒介相互作用的工具。

Evaluation of two artificial infection methods of live ticks as tools for studying interactions between tick-borne viruses and their tick vectors.

机构信息

Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.

INRAE, Anses, Ecole Nationale Vétérinaire d'Alfort, UMR VIROLOGIE, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.

出版信息

Sci Rep. 2022 Jan 11;12(1):491. doi: 10.1038/s41598-021-04498-9.

DOI:10.1038/s41598-021-04498-9
PMID:35017574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8752753/
Abstract

Up to 170 tick-borne viruses (TBVs) have been identified to date. However, there is a paucity of information regarding TBVs and their interaction with respective vectors, limiting the development of new effective and urgently needed control methods. To overcome this gap of knowledge, it is essential to reproduce transmission cycles under controlled laboratory conditions. In this study we assessed an artificial feeding system (AFS) and an immersion technique (IT) to infect Ixodes ricinus ticks with tick-borne encephalitis (TBE) and Kemerovo (KEM) virus, both known to be transmitted predominantly by ixodid ticks. Both methods permitted TBEV acquisition by ticks and we further confirmed virus trans-stadial transmission and onward transmission to a vertebrate host. However, only artificial feeding system allowed to demonstrate both acquisition by ticks and trans-stadial transmission for KEMV. Yet we did not observe transmission of KEMV to mice (IFNAR or BALB/c). Artificial infection methods of ticks are important tools to study tick-virus interactions. When optimally used under laboratory settings, they provide important insights into tick-borne virus transmission cycles.

摘要

迄今为止，已鉴定出多达 170 种蜱传病毒（TBVs）。然而，关于 TBVs 及其与各自载体的相互作用的信息很少，这限制了新的有效和急需的控制方法的发展。为了克服这一知识差距，有必要在受控的实验室条件下再现传播周期。在这项研究中，我们评估了一种人工喂养系统（AFS）和一种浸泡技术（IT），以感染蜱传脑炎（TBE）和克麦罗沃（KEM）病毒的蜱，这两种病毒都已知主要通过硬蜱传播。这两种方法都允许蜱获得 TBEV，我们进一步证实了病毒经代传播和向脊椎动物宿主的传播。然而，只有人工喂养系统允许同时证明 KEMV 的获得和经代传播。然而，我们没有观察到 KEMV 向 IFNAR 或 BALB/c 小鼠的传播。蜱的人工感染方法是研究蜱-病毒相互作用的重要工具。当在实验室环境中最佳使用时，它们为蜱传病毒的传播周期提供了重要的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/fab65b5fb1bc/41598_2021_4498_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/f802d9787c29/41598_2021_4498_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/d925cf5600f7/41598_2021_4498_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/7961e7ad8b10/41598_2021_4498_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/fab65b5fb1bc/41598_2021_4498_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/f802d9787c29/41598_2021_4498_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/ecf8e94904a1/41598_2021_4498_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/d925cf5600f7/41598_2021_4498_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/7961e7ad8b10/41598_2021_4498_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f953/8752753/fab65b5fb1bc/41598_2021_4498_Fig5_HTML.jpg

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