Department of Zoology, Abdul Wali Khan University Mardan, Mardan, Pakistan.
Department of Zoology, GC Women University Sialkot, Sialkot, Pakistan.
Braz J Biol. 2022 Jan 5;84:e251747. doi: 10.1590/1519-6984.251747. eCollection 2022.
Fish is the main source of animal protein for human diet. The aim of this study was to find out prevalence of pathogenic bacteria of two selected economically important fish of Pakistan namely Mahseer (Tor putitora) and Silver carp (Hypophthalmichthys molitrix). Live fish samples from hatcheries and dead fish samples from different markets of study area were randomly collected. The fish samples were analyzed for isolation, identification and prevalence of bacteria. The isolated bacteria from study fish were identified through biochemical test and about 10 species of pathogenic bacteria were identified including the pathogenic bacteria to human and fish namely, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus iniae, Serratia spp. Citrobacter spp. Stenotrophomonas spp. Bacillus spp. and Salmonella spp. The bacterial percentage frequency of occurrence in Silver carp and Mahseer fish showed Pseudomonas aeruginosa 21.42%, Staphylococcus epidermidis 17.85%, Escherichia coli 11.90%, Staphylococcus aureus 9.52%, Citrobacter spp. 9.52%, Serratia spp. 8.33%, Streptococcus iniae 7.14%, Stenotrophomonas spp. 5.95%, Bacillus spp. 4.76% and Salmonella spp. 3.57%. The study revealed that Fish samples of Mahseer and Silver carp that were collected from markets have found more isolates (10 bacterial species) than did the fresh fish pond samples (03 bacterial species) of hatcheries. The occurrence of pathogenic bacteria in study fish showed risk factor for public health consumers.
鱼类是人类饮食中动物蛋白质的主要来源。本研究旨在了解巴基斯坦两种经济重要鱼类，即哲罗鱼（Tor putitora）和白鲢（Hypophthalmichthys molitrix）的病原菌流行情况。从孵化场随机采集活鱼样本和研究区域不同市场的死鱼样本。对鱼样进行分离、鉴定和细菌流行情况分析。从研究鱼类中分离出的细菌通过生化试验进行鉴定，共鉴定出 10 种病原菌，包括对人和鱼类致病的细菌，如铜绿假单胞菌、大肠杆菌、金黄色葡萄球菌、表皮葡萄球菌、海豚链球菌、沙雷氏菌属、柠檬酸杆菌属、寡养单胞菌属、芽孢杆菌属和沙门氏菌属。白鲢和哲罗鱼中细菌出现的百分比频率显示，铜绿假单胞菌为 21.42%，表皮葡萄球菌为 17.85%，大肠杆菌为 11.90%，金黄色葡萄球菌为 9.52%，柠檬酸杆菌属为 9.52%，沙雷氏菌属为 8.33%，海豚链球菌为 7.14%，寡养单胞菌属为 5.95%，芽孢杆菌属为 4.76%，沙门氏菌属为 3.57%。研究表明，从市场采集的哲罗鱼和白鲢鱼样本比孵化场新鲜鱼塘样本（03 种细菌）发现更多的分离株（10 种细菌）。研究鱼类中病原菌的出现对公众健康消费者构成了风险因素。
