Xue Yumeng, Lee Junmin, Kim Han-Jun, Cho Hyun-Jong, Zhou Xingwu, Liu Yaowen, Tebon Peyton, Hoffman Tyler, Qu Moyuan, Ling Haonan, Jiang Xing, Li Zhikang, Zhang Shiming, Sun Wujin, Ahadian Samad, Dokmeci Mehmet R, Lee KangJu, Khademhosseini Ali
Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles , Los Angeles, California 90095, United States.
Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.
ACS Appl Bio Mater. 2020 Oct 19;3(10):6908-6918. doi: 10.1021/acsabm.0c00802. Epub 2020 Oct 7.
Fluorescent nanomaterials have been widely used in biological imaging due to their selectivity, sensitivity, and noninvasive nature. These characteristics make the materials suitable for real-time and in situ imaging. However, further development of highly biocompatible nanosystems with long-lasting fluorescent intensity and photostability is needed for advanced bioimaging. We have used electrospraying to generate gelatin methacryloyl (GelMA)-based fluorescent nanoparticles (NPs) with chemically conjugated rhodamine B (RB). The extent of conjugation can be controlled by varying the mass ratio of RB and GelMA precursors to obtain RB-conjugated GelMA (RB-GelMA) NPs with optimal fluorescent properties and particle size. These NPs exhibited superior biocompatibility when compared with pure RB in in vitro cell viability and proliferation assays using multiple cell types. Moreover, RB-GelMA NPs showed enhanced cell internalization and improved brightness compared with unconjugated RB. Our experiments demonstrate that engineered RB-GelMA NPs can be used as a biocompatible fluorescent label for bioimaging.
荧光纳米材料因其选择性、敏感性和非侵入性，已被广泛应用于生物成像。这些特性使该材料适用于实时和原位成像。然而，为了实现先进的生物成像，需要进一步开发具有持久荧光强度和光稳定性的高生物相容性纳米系统。我们利用电喷雾法制备了基于甲基丙烯酰化明胶（GelMA）的荧光纳米颗粒（NPs），其化学偶联了罗丹明B（RB）。通过改变RB和GelMA前体的质量比，可以控制偶联程度，从而获得具有最佳荧光特性和粒径的RB偶联GelMA（RB-GelMA）纳米颗粒。在使用多种细胞类型的体外细胞活力和增殖试验中，与纯RB相比，这些纳米颗粒表现出优异的生物相容性。此外，与未偶联的RB相比，RB-GelMA纳米颗粒表现出增强的细胞内化和更高的亮度。我们的实验表明，工程化的RB-GelMA纳米颗粒可作为生物成像的生物相容性荧光标记物。
