a Department of Biomaterials, Faculty of Biomedical Engineering , Amirkabir University of Technology , Tehran , Iran.
b Department of Pharmacology, School of Medicine , Shahid Beheshti University of Medical Sciences , Tehran , Iran.
Artif Cells Nanomed Biotechnol. 2018 Dec;46(8):1799-1808. doi: 10.1080/21691401.2017.1392970. Epub 2017 Oct 24.
The cellular microenvironment plays a crucial role in improving cell response and function of an engineered tissue. Scaffolds mimicking the native ECM and capable of releasing growth factors are great candidates for tissue engineering applications. Gelatin methacryloyl (GelMA) hydrogel, a photocrosslinkable biomaterial possessing tunable properties, has been widely used in tissue engineering. It has been suggested that incorporating micro/nano carriers in GelMA could provide a sustained release of growth factors. Specifically, chitosan nanoparticles can be used for growth factor delivery due to its biocompatibility, easy method of synthesis, and preventing the biomolecule from degradation. In this study, GelMA/chitosan nanoparticles composite hydrogel was developed to deliver an angiogenic growth factor (bFGF). The hydrogel was prepared by photopolymerization and its chemical and physical properties were characterized. Its degradation and swelling characteristics were also evaluated. The size of nanoparticles was evaluated and the profile of bFGF release from the hydrogel and its effect on the viability of fibroblast cells was studied. The results showed that GelMA/chitosan nanoparticles can significantly promote cell proliferation due to its biocompatible structure and providing a sustained profile of bFGF release. This hydrogel scaffold can be used for efficient delivery of bFGF in various applications and especially for angiogenesis.
细胞微环境在改善工程化组织中的细胞反应和功能方面起着至关重要的作用。模仿天然细胞外基质并能够释放生长因子的支架是组织工程应用的理想候选材料。明胶甲基丙烯酰（GelMA）水凝胶是一种光交联的生物材料，具有可调节的特性，已广泛应用于组织工程中。有研究表明，在 GelMA 中加入微/纳米载体可以提供生长因子的持续释放。具体来说，壳聚糖纳米粒子由于其生物相容性、易于合成的方法以及防止生物分子降解的特性，可用于生长因子的递送。在本研究中，开发了 GelMA/壳聚糖纳米粒子复合水凝胶来递送血管生成生长因子（bFGF）。通过光聚合制备水凝胶，并对其化学和物理性质进行了表征。还评估了其降解和溶胀特性。评估了纳米粒子的大小，并研究了 bFGF 从水凝胶中的释放及其对成纤维细胞活力的影响。结果表明，GelMA/壳聚糖纳米粒子由于其生物相容性结构和提供 bFGF 持续释放的特性，可以显著促进细胞增殖。这种水凝胶支架可用于各种应用中 bFGF 的有效递送，特别是用于血管生成。
