Wu Chih-Yu, Guo Chin-Lin, Yang Yen-Ching, Huang Chao-Wei, Zeng Jun-Yu, Guan Zhen-Yu, Chiang Yu-Chih, Wang Peng-Yuan, Chen Hsien-Yeh
Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
Molecular Imaging Center, National Taiwan University, Taipei 10617, Taiwan.
ACS Appl Bio Mater. 2020 Oct 19;3(10):7193-7201. doi: 10.1021/acsabm.0c00995. Epub 2020 Oct 6.
A scaffold was fabricated to synergistically encapsulate living human adipose-derived stem cells (hASCs) and platelet-rich plasma (PRP) based on a vapor-phase sublimation and deposition process. During the process, ice templates were prepared using sterile water as the solvent and were used to accommodate the sensitive living cells and PRP molecules. Under controlled processing conditions, the ice templates underwent vapor sublimation to evaporate water molecules, while at the same time, vapor-phase deposition of poly--xylylene (Parylene, USP Class VI highly biocompatible) occurred to replace the templates, and the final construction yielded a scaffold with Parylene as the matrix, with simultaneously encapsulated living hASCs and PRP molecules. Evaluation of the fabricated synergistic scaffold for the proliferation activities toward the encapsulated hASCs indicated significant augmentation of cell proliferation contributed by the PRP ingredients. In addition, osteogenic activity in the early stage by alkaline phosphatase expression and later stage with calcium mineralization indicated significant enhancement toward osteogenetic differentiation of the encapsulated hASCs, which were guided by the PRP molecules. By contrast, examinations of adipogenic activity by lipid droplet formation revealed an inhibition of adipogenesis with decreased intracellular lipid accumulation, and a statistically significant downregulation of adipogenic differentiation was postulated for the scaffold products when compared to the osteogenetic results and the control experiments. The reported fabrication method featured a clean and simple process to construct scaffolds that combined delicate living hASCs and PRP molecules inside the structure. The resultant synergistic scaffold and the selected commercially available hASCs and PRP are emerging as tissue engineering tools that provide multifunctionality for tissue repair and regeneration.
基于气相升华和沉积工艺制备了一种支架，用于协同封装活的人脂肪来源干细胞（hASC）和富血小板血浆（PRP）。在此过程中，以无菌水为溶剂制备冰模板，用于容纳敏感的活细胞和PRP分子。在可控的加工条件下，冰模板经历气相升华以蒸发水分子，同时发生聚对二甲苯（聚对二甲苯，美国药典VI类高生物相容性）的气相沉积以取代模板，最终构建出以聚对二甲苯为基质的支架，同时封装了活的hASC和PRP分子。对制备的协同支架对封装的hASC增殖活性的评估表明，PRP成分显著促进了细胞增殖。此外，通过碱性磷酸酶表达在早期和成骨矿化在后期的成骨活性表明，PRP分子引导下，封装的hASC的成骨分化显著增强。相比之下，通过脂滴形成对成脂活性的检测显示，细胞内脂质积累减少，成脂作用受到抑制，与成骨结果和对照实验相比，推测支架产品的成脂分化有统计学意义的下调。所报道的制造方法具有清洁简单的工艺，可构建在结构内部结合了精细的活hASC和PRP分子的支架。所得的协同支架以及选定的市售hASC和PRP正在成为为组织修复和再生提供多功能性的组织工程工具。
