Departamento de Química, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Santiago, Chile.
Instituto de Ciencias Químicas Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, El Llano Subercaseaux 2801, San Miguel, Santiago 8910060, Chile.
Int J Pharm. 2022 Jan 5;611:121292. doi: 10.1016/j.ijpharm.2021.121292. Epub 2021 Nov 13.
The prevalence of chronic and acute wounds, as well as the complexity of their treatment represent a great challenge for health systems around the world. In this context, the development of bioactive wound dressings that release active agents to prevent infections and promote wound healing, appears as the most promising solution. In this work, we develop an antibacterial and biocompatible wound dressing material made from coaxial electrospun fibers of poly(styrene-co-maleic anhydride) and poly(vinyl alcohol) (PSMA@PVA). The coaxial configuration of the fibers consists of a shell of poly (styrene-co-maleic anhydride) containing a variable concentration of silver nanoparticles (AgNPs) 0.1-0.6 wt% as antibacterial agent, and a core of PVA containing 1 wt% allantoin as healing agent. The fibers present diameters between 0.72 and 1.7 µm. The release of Ag in a physiological medium was studied for 72 h, observing a burst release during the first 14 h and then a sustained and controlled release during the remaining 58 h. Allantoin release curves showed significant release only after 14 h. The meshes showed an antibacterial activity against Pseudomonas aeruginosa and Bacillus subtilis that correlates with the amount of AgNPs incorporated and the release rate of Ag. Indeed, meshes containing 0.3 and 0.6 wt% of AgNPs showed a 99.99% inhibition against both bacteria. The adherence and cell viability of the meshes were evaluated in mouse embryonic fibroblasts NIH/3T3, observing a significant increase in cell viability after 72 h of incubation accompanied by a reduced adhesion of fibroblasts that decreased in the presence of the active agents. These results show that the material prepared here is capable of significantly promoting fibroblast cell proliferation but without strong adherence, which makes it an ideal material for wound dressings with non-adherent characteristics and with potential for wound healing.
慢性和急性伤口的患病率以及其治疗的复杂性对全球卫生系统构成了巨大挑战。在这种情况下，开发能够释放活性物质以预防感染和促进伤口愈合的生物活性伤口敷料成为最有前途的解决方案。在这项工作中，我们开发了一种由聚（苯乙烯-共-马来酸酐）和聚乙烯醇（PSMA@PVA）同轴电纺纤维制成的抗菌且生物相容的伤口敷料材料。纤维的同轴结构由含有不同浓度银纳米粒子（AgNPs）0.1-0.6 wt% 的聚（苯乙烯-共-马来酸酐）外壳组成，作为抗菌剂，以及含有 1 wt% 尿囊素的 PVA 核作为愈合剂。纤维的直径在 0.72 至 1.7 µm 之间。在生理介质中研究了 Ag 的释放，观察到在最初的 14 小时内有突释，然后在剩余的 58 小时内有持续和控制的释放。只有在 14 小时后，尿囊素的释放曲线才显示出显著的释放。该网孔对铜绿假单胞菌和枯草芽孢杆菌表现出抗菌活性，这与 AgNPs 的含量和 Ag 的释放速率有关。实际上，含有 0.3 和 0.6 wt% AgNPs 的网孔对两种细菌的抑制率均达到 99.99%。通过评估细胞在网孔上的黏附和存活能力，发现鼠胚胎成纤维细胞 NIH/3T3 在孵育 72 小时后细胞活力显著增加，同时伴随成纤维细胞黏附减少，而活性物质的存在会进一步减少细胞黏附。这些结果表明，我们制备的材料能够显著促进成纤维细胞的增殖，但不会强烈黏附，使其成为具有非黏附特性和潜在伤口愈合能力的理想伤口敷料材料。
