Zandi Shafagh Reza, Shen Joanne X, Youhanna Sonia, Guo Weijin, Lauschke Volker M, van der Wijngaart Wouter, Haraldsson Tommy
Department of Physiology and Pharmacology, Karolinska Institutet, 171 77 Stockholm, Sweden.
Division of Micro- and Nanosystems, KTH Royal Institute of Technology, 100 44 Stockholm, Sweden.
ACS Appl Bio Mater. 2020 Dec 21;3(12):8757-8767. doi: 10.1021/acsabm.0c01087. Epub 2020 Nov 10.
High-aspect-ratio and hierarchically nanostructured surfaces are common in nature. Synthetic variants are of interest for their specific chemical, mechanic, electric, photonic, or biologic properties but are cumbersome in fabrication or suffer from structural collapse. Here, we replicated and directly biofunctionalized robust, large-area, and high-aspect-ratio nanostructures by nanoimprint lithography of an off-stoichiometric thiol-ene-epoxy polymer. We structured-in a single-step process-dense arrays of pillars with a diameter as low as 100 nm and an aspect ratio of 7.2; holes with a diameter of 70 nm and an aspect ratio of >20; and complex hierarchically layered structures, all with minimal collapse and defectivity. We show that the nanopillar arrays alter mechanosensing of human hepatic cells and provide precise spatial control of cell attachment. We speculate that our results can enable the widespread use of high-aspect-ratio nanotopograhy applications in mechanics, optics, and biomedicine.
高纵横比和分层纳米结构的表面在自然界中很常见。合成变体因其特定的化学、机械、电学、光子学或生物学特性而备受关注，但在制造过程中很麻烦，或者会出现结构坍塌。在这里，我们通过对非化学计量硫醇-烯-环氧聚合物进行纳米压印光刻，复制并直接对坚固、大面积和高纵横比的纳米结构进行生物功能化。我们在一步法中构建了直径低至100nm、纵横比为7.2的密集柱阵列；直径为70nm、纵横比大于20的孔；以及复杂的分层结构，所有这些结构的坍塌和缺陷都最小。我们表明，纳米柱阵列改变了人类肝细胞的机械传感，并提供了细胞附着的精确空间控制。我们推测，我们的结果能够使高纵横比纳米拓扑应用在力学、光学和生物医学中得到广泛应用。
