The Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, and Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China.
The Education Ministry Key Lab of Resource Chemistry, Joint International Research Laboratory of Resource Chemistry, Ministry of Education, and Shanghai Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Materials Science, Shanghai Normal University, Shanghai, 200234, China.
Biosens Bioelectron. 2022 Apr 1;201:113962. doi: 10.1016/j.bios.2022.113962. Epub 2022 Jan 6.
A novel potential-resolved molecularly imprinted electrochemical luminescence (ECL) immunosensor has been developed for the first time for the dual sensitive detection of markers of acute myocardial infarction (AMI): cardiac troponin I (cTnI) and myoglobin (Mb). In this work, cost-effective and robust molecularly imprinted polymer (MIP) as biomimetic antibody was used to construct the immunosensors through electropolymerization and elution to form polydopamine (PDA)-MIP modified electrode. In the presence of AMI biomarkers, two ECL probes including Ru(bpy)@ MOCs and MoS QDs functionalized by cTnI antibody and Mb aptamer could be specifically captured respectively. And two potential distinct ECL signals will be generated in one potential scan. The intensity of ECL reflects the concentrations of cTnI and Mb. The two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, FT-IR spectrum and UV-Vis diffuse reflectance spectroscopy. The prepared sensor exhibited a wide linear range (0.05-10 ng/mL) and a low detection limit (0.0184 ng/mL for cTnI and 0.0492 ng/mL for Mb). Additionally, the MIP-ECL sensor displayed excellent anti-interference, sensitivity and stability to detect cTnI and Mb. Therefore, it will be conducive to accelerate more precise and credible early diagnosis for AMI.
一种新型的基于电位分辨的分子印迹电化学发光（ECL）免疫传感器首次被开发出来，用于双重敏感检测急性心肌梗死（AMI）标志物：心肌肌钙蛋白 I（cTnI）和肌红蛋白（Mb）。在这项工作中，使用具有成本效益和稳健的分子印迹聚合物（MIP）作为仿生抗体，通过电聚合和洗脱来构建免疫传感器，以形成聚多巴胺（PDA）-MIP 修饰电极。在存在 AMI 生物标志物的情况下，两种 ECL 探针（包括 Ru（bpy）@MOCs 和 MoS QDs）可以通过 cTnI 抗体和 Mb 适体功能化，分别被特异性捕获。并且在一次电位扫描中会产生两个具有明显区别的 ECL 信号。ECL 的强度反映了 cTnI 和 Mb 的浓度。两种 ECL 探针的特性采用场发射扫描电子显微镜、X 射线衍射、FT-IR 光谱和紫外-可见漫反射光谱进行了表征。所制备的传感器表现出宽的线性范围（0.05-10ng/mL）和低的检测限（cTnI 为 0.0184ng/mL，Mb 为 0.0492ng/mL）。此外，MIP-ECL 传感器对检测 cTnI 和 Mb 具有出色的抗干扰性、灵敏度和稳定性。因此，这将有助于加速更精确和可靠的 AMI 早期诊断。
