Hol P R, Snel F W, Draaijer M, Gruys E
Department of Veterinary Pathology, University of Utrecht, The Netherlands.
J Comp Pathol. 1987 Nov;97(6):677-85. doi: 10.1016/0021-9975(87)90079-x.
The acute phase SAA response was studied in hamsters. An SAA-stimulating factor (SAASF) was detected in the early acute phase blood plasma of hamsters which were subcutaneously injected with casein-LPS. The latter is routinely used in our laboratory for amyloid induction in hamsters. Acute (4 h) inflammatory exudates (greater than 80 per cent polymorphonuclear leukocytes) were produced by intraperitoneal injection with either casein-LPS, latex or Freund's incomplete adjuvant. Chronic inflammatory exudate macrophages (greater than 98 per cent) were elicited by intraperitoneal injection with Bacillus Calmette Guérin (BCG). Cells were stimulated in vitro with latex. SAASF was detected in the supernates and lysates of the acute exudate cells but not in those of the chronic peritoneal exudate macrophages. Lymphocyte activating factor (LAF), however, was evidently present in the latter samples, indicating that SAASF and LAF (IL-1) are functionally different substances in hamsters.
在仓鼠中研究了急性期血清淀粉样蛋白A（SAA）反应。在皮下注射酪蛋白-脂多糖的仓鼠急性期早期血浆中检测到一种SAA刺激因子（SAASF）。后者在我们实验室中常用于诱导仓鼠产生淀粉样变。通过腹腔注射酪蛋白-脂多糖、乳胶或弗氏不完全佐剂可产生急性（4小时）炎症渗出物（多形核白细胞大于80%）。通过腹腔注射卡介苗（BCG）可诱导产生慢性炎症渗出物巨噬细胞（大于98%）。用乳胶在体外刺激细胞。在急性渗出细胞的上清液和裂解物中检测到SAASF，但在慢性腹膜渗出巨噬细胞中未检测到。然而，淋巴细胞激活因子（LAF）明显存在于后者的样本中，表明SAASF和LAF（白细胞介素-1）在仓鼠中是功能不同的物质。
