Li F, Shu C D, Li Z M
Artificial Liver Research Laboratory, Chongqing University of Medical Sciences, Sichuan, China.
Biomater Artif Cells Artif Organs. 1987;15(1):215-28.
Blood of rats and guinea pigs on cross-linked agarose coated activated charcoal (CAAC-II) hemoperfusion was analyzed over a follow-up period of 48 hours for the capacity of T-lymphocyte to transform under the stimulation of mitogen (PHA) in vivo and in vitro respectively. In the rate hemoperfusion, the in vivo T-lymphocyte transformation and lymphocyte count of peripheral blood were involved. In guinea pigs, the lymphocyte cultures were labelled with 3H-TdR and the radioactive incorporation was measured on the liquid scintillation counter. Our results revealed that the T-lymphocyte transformation function remained unchanged after 60-minute CAAC-II hemoperfusion although there was a transient and incomplete suppression of the T-lymphocyte transformation function both in vivo and in vitro immediately after the anesthesia and operation with blood vessel cannulation. The peripheral blood lymphocyte count also remained stable during the 60-minute CAAC-II hemoperfusion on rats and guinea pigs. We concluded that the charcoal hemoperfusion can be used as an important method of treatment for drug intoxication, uremia and hepatic coma with no harm to the body's immune function.
在交联琼脂糖包被的活性炭（CAAC-II）血液灌流48小时的随访期内，分别分析大鼠和豚鼠血液中T淋巴细胞在体内和体外有丝分裂原（PHA）刺激下的转化能力。在大鼠血液灌流中，涉及体内T淋巴细胞转化和外周血淋巴细胞计数。在豚鼠中，用3H-TdR标记淋巴细胞培养物，并在液体闪烁计数器上测量放射性掺入。我们的结果显示，尽管在麻醉和血管插管手术后，体内和体外的T淋巴细胞转化功能立即出现短暂且不完全的抑制，但在60分钟的CAAC-II血液灌流后，T淋巴细胞转化功能保持不变。在大鼠和豚鼠60分钟的CAAC-II血液灌流期间，外周血淋巴细胞计数也保持稳定。我们得出结论，活性炭血液灌流可作为治疗药物中毒、尿毒症和肝昏迷的重要方法，且对机体免疫功能无害。
