Khimji P L, Miles A A
Br J Exp Pathol. 1978 Apr;59(2):137-47.
Preparations of catechols from ethyl acetate extracts of cultures of Klebsiellae in a low-iron medium contained iron-chelators whose potency was measured by the reversal of the bacteristasis of Escherichia coli and klebsiellae in unheated horse serum, and of the growth-inhibition of these two organisms by ethylene diamine di-orthohydroxyphenyl acetic acid (EDDA). As revealed by in situ tests of paper chromatograms, there was a multiplicity of biologically active chelators in the preparations. Catechols from strains both of high and low virulence for guinea-pigs enhanced the skin infectivity of most of the 10 Klebsiella strains tested. The enhancement was roughly proportional to iron-enhanceability with the 6 iron-enhanceable (E+) strains, though not as great as that by iron. But of the 4 (Eo) strains not enhanceable by iron, two were moderately enhanced by the catechols. The Streptomyces iron-chelator desferrioxamine B also enhanced infectivity, again roughly in proportion to the iron enhanceability of the strains; though one Eo strain was substantially enhanced. The synthetic iron-chelator EDDA did not enhance infection.
在低铁培养基中，用克雷伯氏菌培养物的乙酸乙酯提取物制备儿茶酚，其中含有铁螯合剂，其效力通过在未加热马血清中逆转大肠杆菌和克雷伯氏菌的抑菌作用以及通过乙二胺二邻羟基苯基乙酸（EDDA）对这两种菌的生长抑制作用来测定。纸色谱原位试验表明，制剂中存在多种具有生物活性的螯合剂。对豚鼠高毒力和低毒力菌株的儿茶酚增强了所测试的10株克雷伯氏菌中大多数菌株的皮肤感染性。对于6株可被铁增强（E +）的菌株，这种增强作用大致与铁增强能力成比例，尽管不如铁的增强作用大。但是在4株不能被铁增强（Eo）的菌株中，有两株被儿茶酚适度增强。链霉菌铁螯合剂去铁胺B也增强了感染性，同样大致与菌株的铁增强能力成比例；尽管有一株Eo菌株被显著增强。合成铁螯合剂EDDA没有增强感染。
