VISAFELab, Department of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food Technology, IATA-CSIC, Av. Agustín Escardino 7, Paterna, Valencia 46980, Spain.
Instituto de Biomedicina de Valencia (IBV-CSIC), C/ Jaume Roig, 11, Valencia 46010, Spain.
Water Res. 2022 Mar 1;211:118007. doi: 10.1016/j.watres.2021.118007. Epub 2021 Dec 24.
Wastewater-based epidemiology (WBE) has proven to be an effective tool for epidemiological surveillance of SARS-CoV-2 during the current COVID-19 pandemic. Furthermore, combining WBE together with high-throughput sequencing techniques can be useful for the analysis of SARS-CoV-2 viral diversity present in a given sample. The present study focuses on the genomic analysis of SARS-CoV-2 in 76 sewage samples collected during the three epidemiological waves that occurred in Spain from 14 wastewater treatment plants distributed throughout the country. The results obtained demonstrate that the metagenomic analysis of SARS-CoV-2 in wastewater allows the detection of mutations that define the B.1.1.7 lineage and the ability of the technique to anticipate the detection of certain mutations before they are detected in clinical samples. The study proves the usefulness of sewage sequencing to track Variants of Concern that can complement clinical testing to help in decision-making and in the analysis of the evolution of the pandemic.
基于污水的流行病学（WBE）已被证明是当前 COVID-19 大流行期间对 SARS-CoV-2 进行流行病学监测的有效工具。此外，将 WBE 与高通量测序技术相结合，可用于分析给定样本中存在的 SARS-CoV-2 病毒多样性。本研究重点对来自西班牙全国分布的 14 个污水处理厂的 76 个污水样本中 SARS-CoV-2 的基因组进行分析。研究结果表明，对污水中 SARS-CoV-2 的宏基因组分析可检测到定义 B.1.1.7 谱系的突变，并且该技术能够在临床样本中检测到某些突变之前提前检测到这些突变。该研究证明了对污水进行测序来追踪关注变异株的实用性，这种方法可以与临床检测互补，以帮助决策，并分析大流行的演变。
