Laboratory of Biochemistry, Research and Development Department, APIVITA S.A., Industrial Park of Markopoulo Mesogaias, Markopoulo Attiki, 19003, Athens, Greece.
In Vitro Plant-Tech AB, Geijersg 4B, 21618, Limhamn, Sweden.
Sci Rep. 2020 Nov 5;10(1):19213. doi: 10.1038/s41598-020-76169-0.
Natural ingredients have been used to improve the state of health in humans. The genus Paeonia has been studied only limited yet it's reported to have many activities such as antioxidant and anti-inflammatory. To this context, here we focused on an endemic Paeonia species in Attica. This study aims to present the development of the Paeonia mascula subsp. hellenica callus extract and its pleiotropic bioactivity on human primary keratinocytes exploring its potential application as an active agent in skin-related products. This extract showed a high scavenging activity with high phenolic content and an interesting metabolic profile. At a molecular level, the study on the transcript accumulation of genes revealed that this extract exhibits in vitro skin-related protection properties by mediating mitochondrial energy, cell proliferation, immune and inflammatory response and positively regulates genes involved in epidermal and in stratum corneum function. Besides, the extract is proven not skin irritant on reconstructed human skin model. These findings indicate that the specific P. mascula subsp. hellenica extract possesses significant in vitro protection activity on human epidermis and provides new insights into its beneficial role in skin confirming that the advent of biotechnology contribution the past few decades.
天然成分被用于改善人类的健康状况。虽然对牡丹属的研究还很有限，但据报道它具有许多活性，如抗氧化和抗炎作用。在这方面，我们专注于研究阿提卡特有的牡丹品种。本研究旨在展示雄性牡丹亚种的愈伤组织提取物的开发及其对人原代角质形成细胞的多种生物活性，探索其作为皮肤相关产品中活性成分的潜在应用。该提取物具有较高的酚类含量和有趣的代谢特征，表现出较高的清除活性。在分子水平上，对基因转录积累的研究表明，该提取物通过调节线粒体能量、细胞增殖、免疫和炎症反应，表现出体外皮肤保护特性，并正向调节与表皮和角质层功能相关的基因。此外，该提取物在重建的人体皮肤模型上被证明不会引起皮肤刺激。这些发现表明，特定的雄性牡丹亚种提取物对人表皮具有显著的体外保护活性，并为其在皮肤中的有益作用提供了新的见解，证实了生物技术在过去几十年中的贡献。
