Cullen D C, Brown R G, Lowe C R
Institute of Biotechnology, University of Cambridge, UK.
Biosensors. 1987;3(4):211-25. doi: 10.1016/0265-928x(87)85002-2.
The sensitivity of surface plasmon resonance techniques to changes in local interfacial refractive index has been exploited to detect immuno-complex formation in two model biochemical systems. A gold-coated diffraction grating has been used to excite surface plasmons at the gold/solution interface to which either human immunoglobulin G or the immunoglobulin fraction of sheep antiserum to human serum albumin was physically adsorbed. The complementary proteins, either affinity purified goat anti-human-IgG IgG or human serum albumin was subsequently specifically bound by immuno-complex formation. The binding reactions could be followed with respect to time.
表面等离子体共振技术对局部界面折射率变化的敏感性已被用于检测两个模型生化系统中的免疫复合物形成。一个镀金的衍射光栅已被用于在金/溶液界面激发表面等离子体，人免疫球蛋白G或羊抗人血清白蛋白抗血清的免疫球蛋白部分被物理吸附在该界面上。互补蛋白，即亲和纯化的山羊抗人IgG IgG或人血清白蛋白，随后通过免疫复合物形成特异性结合。结合反应可以随时间进行跟踪。
