Jin G, Tengvall P, Lundström I, Arwin H
Department of Physics and Measurement Technology, Linköping University, Sweden.
Anal Biochem. 1995 Nov 20;232(1):69-72. doi: 10.1006/abio.1995.9959.
A new type of optical biosensor system is proposed and its application to detection of antigen-antibody complexes on a silicon substrate is demonstrated. The biosensor system is utilizing specificities of biomolecular interactions in combination with protein patterned surfaces. Visualization of the thickness distributions of thin layers (protein patterns) on a surface is achieved by using imaging ellipsometry. This technique uses a CCD camera whereby an ellipsometric analysis of a large surface can be made. The biosensor system has the advantages of high spatial resolution, fast data acquisition, and simplicity in use.
提出了一种新型的光学生物传感器系统，并展示了其在硅基片上检测抗原-抗体复合物的应用。该生物传感器系统利用生物分子相互作用的特异性与蛋白质图案化表面相结合。通过使用成像椭偏仪实现了表面薄层（蛋白质图案）厚度分布的可视化。该技术使用电荷耦合器件（CCD）相机，从而可以对大面积表面进行椭偏分析。该生物传感器系统具有高空间分辨率、快速数据采集和使用简单的优点。
