Mattson D H, Roos R P, Arnason B G
Ann Neurol. 1981 Jan;9(1):34-41. doi: 10.1002/ana.410090107.
Isoelectric focusing (IEF) and agar gel electrophoresis (AGE) were used to examine cerebrospinal fluid (CSF) and sera from patients with multiple sclerosis (MS) and subacute sclerosing panencephalitis (SSPE). All 15 SSPE samples and 29 of the 33 MS CSF samples showed oligoclonal IgG bands by AGE and IEF. Serum bands were more frequent in SSPE than in MS and were more commonly detected by IEF than by AGE. In MS CSF the number of bands on IEF correlated with: (1) disease duration, (2) CSF IgG, (3) CSF IgG/albumin ratio, and (4) central nervous system IgG synthesis. Serial studies revealed increases in IEF band number in 4 of 10 MS and 3 of 5 SSPE CSF specimens; fluctuations in band intensity were also noted. Densitometric scans of CSF IEF gels showed high, sharply angled IgG band peaks in SSPE; the CSF band peaks in MS were flatter and had higher background IgG. The SSPE pattern could be made to resemble the MS pattern through addition of normal polyclonal IgG to SSPE CSF. These findings suggest that in addition to oligoclonal IgG, polyclonal IgG is synthesized locally within the central nervous system in MS.
采用等电聚焦（IEF）和琼脂凝胶电泳（AGE）检测多发性硬化症（MS）和亚急性硬化性全脑炎（SSPE）患者的脑脊液（CSF）和血清。所有15份SSPE样本以及33份MS脑脊液样本中的29份，通过AGE和IEF检测均显示出寡克隆IgG条带。SSPE患者血清条带出现的频率高于MS患者，且通过IEF检测比AGE更常检测到血清条带。在MS脑脊液中，IEF上条带的数量与以下因素相关：（1）病程，（2）脑脊液IgG，（3）脑脊液IgG/白蛋白比值，以及（4）中枢神经系统IgG合成。系列研究显示，10份MS脑脊液标本中的4份以及5份SSPE脑脊液标本中的3份，IEF条带数量增加；还注意到条带强度存在波动。脑脊液IEF凝胶的光密度扫描显示，SSPE中IgG条带峰高且呈锐角；MS中的脑脊液条带峰较平缓，背景IgG较高。通过向SSPE脑脊液中添加正常多克隆IgG，可使SSPE的图谱类似于MS的图谱。这些发现表明，除了寡克隆IgG外，MS患者中枢神经系统内还局部合成多克隆IgG。
