Lv Jinhua, Dong Tianyu, Zhang Yanping, Ku Yu, Zheng Ting, Jia Haifeng, Fang Jinggui
College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
Suzhou Polytechnic Institute of Agriculture, Suzhou, 215008, Jiangsu, People's Republic of China.
Plant Cell Rep. 2022 Apr;41(4):935-946. doi: 10.1007/s00299-022-02829-2. Epub 2022 Jan 19.
Hormone treatment enhanced the content of osmotic substances under high-temperature conditions. The effect of ABA and BR treated separately is better than treated together. To determine the effect of abscisic acid (ABA), brassinolide (BR) and ABA + BR on grape quality under high-temperature stress, various metabolites were analyzed. Compared with the control (CK), DL-tryptophan, D-raffinose, geniposidic acid, dodecanedioic acid and polyphenols were found to be higher after ABA treatment. After BR treatment, amino acids and poricoic acid B were higher than in CK. And carbohydrates and amino acids were up-regulated after ABA + BR treatment. BR and ABA + BR treatment also induced higher endogenous ABA and epibrassinolide contents. In addition, treated grape had higher soluble solid concentrations and soluble sugar content, and delayed the degradation of middle lamella and microfibrils. Antioxidant and heat shock-related genes were examined, which significantly increased in treated grape. The finding of this study suggested that ABA, BR and ABA + BR are very useful for alleviating high-temperature damage by increasing the accumulation of osmotic adjustment substances, and endogenous hormones content.
激素处理提高了高温条件下渗透物质的含量。单独使用脱落酸（ABA）和油菜素内酯（BR）处理的效果优于两者共同处理。为了确定脱落酸（ABA）、油菜素内酯（BR）以及ABA + BR对高温胁迫下葡萄品质的影响，对各种代谢产物进行了分析。与对照（CK）相比，ABA处理后发现DL-色氨酸、D-棉子糖、京尼平苷酸、十二烷二酸和多酚含量更高。BR处理后，氨基酸和茯苓酸B的含量高于对照。ABA + BR处理后碳水化合物和氨基酸上调。BR和ABA + BR处理还诱导了更高的内源ABA和表油菜素内酯含量。此外，处理后的葡萄具有更高的可溶性固形物浓度和可溶性糖含量，并延缓了中层和微纤丝的降解。对抗氧化和热休克相关基因进行了检测，处理后的葡萄中这些基因显著增加。本研究结果表明，ABA、BR和ABA + BR通过增加渗透调节物质的积累和内源激素含量，对减轻高温伤害非常有用。
