Negrel Lise, Halter David, Wiedemann-Merdinoglu Sabine, Rustenholz Camille, Merdinoglu Didier, Hugueney Philippe, Baltenweck Raymonde
SVQV, Institut National de la Recherche Agronomique, Université de Strasbourg, Colmar, France.
Front Plant Sci. 2018 Mar 21;9:360. doi: 10.3389/fpls.2018.00360. eCollection 2018.
The Oomycete is responsible for downy mildew, which is one of the most damaging grapevine diseases. Due to the strictly biotrophic way of life of , its metabolome is relatively poorly characterized. In this work, we have used a mass spectrometry-based non-targeted metabolomic approach to identify potential -specific metabolites. This has led to the characterization and structural elucidation of compounds belonging to three families of atypical lipids, which are not detected in healthy grapevine tissues. These lipids include ceramides and derivatives of arachidonic and eicosapentaenoic acid, most of which had not been previously described in Oomycetes. Furthermore, we show that these lipids can be detected in -infected tissues at very early stages of the infection process, long before the appearance the first visible symptoms of the disease. Therefore, the potential use of these specific lipids as markers to monitor the development of is discussed.
卵菌纲生物是导致霜霉病的病原体，霜霉病是最具破坏性的葡萄藤病害之一。由于其严格的活体营养生活方式，其代谢组的特征相对较少。在这项工作中，我们使用了基于质谱的非靶向代谢组学方法来鉴定潜在的卵菌纲特异性代谢物。这导致了属于三个非典型脂质家族的化合物的表征和结构解析，这些脂质在健康的葡萄藤组织中未被检测到。这些脂质包括神经酰胺以及花生四烯酸和二十碳五烯酸的衍生物，其中大多数以前在卵菌纲中未曾描述过。此外，我们表明，在感染过程的非常早期阶段，即在疾病首次出现可见症状之前很久，就可以在被卵菌纲感染的组织中检测到这些脂质。因此，讨论了这些特定脂质作为监测卵菌纲发展的标志物的潜在用途。
