Laboratory of Environmental Biotechnology, Federal University of Latin-American Integration - UNILA, Av. Tarquínio Joslin Dos Santos, 1000-Jd Universitário, G11, Foz do Iguaçu, Paraná, 85870-901, Brazil.
Brazilian Agricultural Research Corporation, Embrapa Meio Ambiente, Jaguariúna, Brazil.
Curr Microbiol. 2022 Jan 20;79(2):70. doi: 10.1007/s00284-021-02752-x.
Urban waste (UW) has caused a series of problems regarding its management. UW comprises domestic, hospital and industrial residues, which makes the destination of this waste a matter of concern, as it may contain a variety of highly toxic environmental polluters. Deactivated dumps can represent sources of contamination of the environment that surround these deposits, harming rivers and inhabiting organisms. Knowledge of the microbial profile of water bodies that can be affected by these toxic residues is essential for the development of alternatives and improvements in treatments applied in rivers and streams. In this sense, this work aimed to analyze the microbial community present in sediments of the Arroio Dourado stream in the municipality of Foz do Iguaçu, a stream located near a deactivated open-air dump. 16S rDNA metabarcoding suggested the dominance of acidogenic bacteria belonging to Acidobacteriota phylum, followed by less abundant phyla Actinobacteriota, Myxococcota, Chloroflexi and a small community of sulfate reducers (Desulfobacteriota). However, more than 50% of amplicon sequence variants (ASVs) were not taxonomically classified. In addition, an expressive abundance was attributed to the genus Anaeromyxobacter, a metabolically versatile group, which can thrive in the presence of polluting compounds present in the deactivated landfill. Thus, a possible stream treatment process can be developed. In addition, culture media can be developed for the recovery of taxonomic groups identified involved in the biodegradation of organic compounds. The results presented expand the knowledge of bacterial diversity in sediment samples recovered from the Arroio Dourado stream.
城市废物（UW）在其管理方面造成了一系列问题。UW 包括家庭、医院和工业废物，这使得这些废物的去向成为一个令人关注的问题，因为它可能含有各种剧毒的环境污染物。已停用的垃圾场可能成为这些垃圾场周围环境污染的来源，危害河流和栖息生物。了解可能受到这些有毒废物影响的水体的微生物特征对于开发替代方案和改进河流和溪流中的处理方法至关重要。在这种情况下，本工作旨在分析位于伊瓜苏市附近已停用的露天垃圾场附近的 Arroio Dourado 溪流沉积物中的微生物群落。16S rDNA 代谢组学表明，属于 Acidobacteriota 门的产酸菌占优势，其次是丰度较低的放线菌门、粘球菌门、绿弯菌门和少量硫酸盐还原菌门（脱硫杆菌门）。然而，超过 50%的扩增子序列变异体（ASV）未进行分类学分类。此外，属 Anaeromyxobacter 的丰度很高，这是一个代谢多样的群体，能够在已停用的垃圾填埋场中存在的污染化合物存在的情况下茁壮成长。因此，可以开发一种可能的溪流处理工艺。此外，可以开发用于回收鉴定的与有机化合物生物降解相关的分类群的培养基。所呈现的结果扩展了从 Arroio Dourado 溪流中回收的沉积物样品中细菌多样性的知识。
