Farian Ewelina, Wójcik-Fatla Angelina
Department of Health Biohazards and Parasitology, Institute of Rural Health, Jaczewskiego 2, 20-090 Lublin, Poland.
Indian J Microbiol. 2022 Mar;62(1):146-151. doi: 10.1007/s12088-021-00966-y. Epub 2021 Aug 15.
Fungi are one of the most widely distributed microorganisms in the environment, including food such as fruits, vegetables and other crops, posing a potential threat to food safety and human health. The aim of this study was to determine the diversity, intensity and drug resistance of potentially pathogenic filamentous fungi isolated from the fresh raspberries ( L.). A total of 50 strains belonging to genera , , , , , , and were tested for drug resistance against 11 antifungals by disc diffusion and gradient strips methods. The average mycological contamination in the examined samples of raspberries amounted to 4.34 log CFU/g. The was isolated from all tested samples, followed by and with a frequency of 61% and 34%, respectively. The highest level of drug resistance was observed for genera and strains recorded a wide variation in drug resistance as revealed by susceptibility with amphotericin B and voriconzole with MICs ranged from 0.5-4 µg/ml and posaconazole with MICs ranging from 3-8 µg/ml. All fungal strains showed 100% resistance to caspofungin, fluconazole and flucytosine with both the methods, and 100% resistance to micafungin and anidulafungin in the gradient strip method.
真菌是环境中分布最广泛的微生物之一,存在于水果、蔬菜和其他农作物等食物中,对食品安全和人类健康构成潜在威胁。本研究旨在确定从新鲜树莓(L.)中分离出的潜在致病性丝状真菌的多样性、强度和耐药性。通过纸片扩散法和梯度条法,对总共50株属于、、、、、、和属的菌株进行了11种抗真菌药物的耐药性测试。所检测的树莓样本中的平均真菌污染量为4.34 log CFU/g。从所有测试样本中均分离出了,其次是和,分离频率分别为61%和34%。属的菌株耐药性水平最高,菌株对两性霉素B和伏立康唑的药敏试验显示耐药性差异很大,其最低抑菌浓度(MIC)范围为0.5 - 4 μg/ml,泊沙康唑的MIC范围为3 - 8 μg/ml。两种方法均显示所有真菌菌株对卡泊芬净、氟康唑和氟胞嘧啶耐药率为100%,梯度条法中对米卡芬净和阿尼芬净耐药率为100%。