雪旺细胞通过Mif/CD74/FOXO1信号通路在体外加速成骨作用。
Schwann Cells Accelerate Osteogenesis via the Mif/CD74/FOXO1 Signaling Pathway In Vitro.
作者信息
Li Jun-Qin, Jiang Hui-Jie, Su Xiu-Yun, Feng Li, Zhan Na-Zhi, Li Shan-Shan, Chen Zi-Jie, Chang Bo-Han, Cheng Peng-Zhen, Yang Liu, Pei Guo-Xian
机构信息
Department of Orthopaedics, Xijing Hospital, Air Force Medical University, Xi'an 710032, China.
Southern University of Science and Technology Hospital, No. 6019 Liuxian Street, Xili Avenue, Nanshan District, Shenzhen 518055, China.
出版信息
Stem Cells Int. 2022 Jan 13;2022:4363632. doi: 10.1155/2022/4363632. eCollection 2022.
Schwann cells have been found to promote osteogenesis by an unclear molecular mechanism. To better understand how Schwann cells accelerate osteogenesis, RNA-Seq and LC-MS/MS were utilized to explore the transcriptomic and metabolic response of MC3T3-E1 to Schwann cells. Osteogenic differentiation was determined by ALP staining. Lentiviruses were constructed to alter the expression of Mif (macrophage migration inhibitory factor) in Schwann cells. Western blot (WB) analysis was employed to detect the protein expression. The results of this study show that Mif is essential for Schwann cells to promote osteogenesis, and its downstream CD74/FOXO1 is also involved in the promotion of Schwann cells on osteogenesis. Further, Schwann cells regulate amino acid metabolism and lipid metabolism in preosteoblasts. These findings unveil the mechanism for Schwann cells to promote osteogenesis where Mif is a key factor.
人们发现施万细胞可通过一种尚不清楚的分子机制促进成骨作用。为了更好地理解施万细胞如何加速成骨，利用RNA测序（RNA-Seq）和液相色谱-串联质谱（LC-MS/MS）来探索MC3T3-E1细胞对施万细胞的转录组和代谢反应。通过碱性磷酸酶（ALP）染色来确定成骨分化。构建慢病毒以改变施万细胞中巨噬细胞迁移抑制因子（Mif）的表达。采用蛋白质免疫印迹（WB）分析来检测蛋白质表达。本研究结果表明，Mif对施万细胞促进成骨至关重要，其下游的CD74/叉头框蛋白O1（FOXO1）也参与施万细胞对成骨的促进作用。此外，施万细胞调节前成骨细胞中的氨基酸代谢和脂质代谢。这些发现揭示了施万细胞促进成骨的机制，其中Mif是关键因素。
Zotero 插件