Löve A, Rydbeck R, Ljungdahl A, Kristensson K, Norrby E
Department of Virology, Karolinska Institute, Stockholm, Sweden.
Microb Pathog. 1986 Apr;1(2):149-58. doi: 10.1016/0882-4010(86)90017-3.
The neurotropic Kilham strain of mumps virus was serially passaged in newborn hamster brains in order to assess possible changes in viral characteristics. Two modes of passage were employed, one with a 4-5 day interval between inoculation and harvest and the other with a 10-12 day interval. After 10 and 8 passages, respectively, two viral variants were isolated which differed in antigen characteristics and in pathogenicity. In Vero cell cultures the variant derived from the short-term passage, designated as RK, showed much greater fusion capacity than the other, designated as SK. The highly fusing variant was highly lethal and caused much more extensive necrosis and grew to higher titers in the brain. With a series of monoclonal antibodies directed against the structural proteins of mumps virus marked differences between the variants could be detected in the nucleocapsid (NP) protein and also slight changes in the hemagglutinin-neuraminidase (HN) and phospho- (P) proteins. Differences were found in the preference of the viral variants to infect various regions of the brain. The RK variant heavily infected the caudate nucleus whereas the SK variant did not. This study demonstrates that different modes of passage can affect characteristics of virion components and disease pattern.
为了评估腮腺炎病毒嗜神经的基尔汉姆株病毒特性可能发生的变化，该病毒在新生仓鼠脑内连续传代。采用了两种传代方式，一种是接种与收获之间间隔4 - 5天，另一种是间隔10 - 12天。分别传代10次和8次后，分离出两种病毒变体，它们在抗原特性和致病性方面存在差异。在非洲绿猴肾细胞（Vero）培养物中，源自短期传代的变体，命名为RK，其融合能力比另一种命名为SK的变体强得多。高融合能力的变体具有高度致死性，会导致更广泛的坏死，并且在脑中生长至更高滴度。利用一系列针对腮腺炎病毒结构蛋白的单克隆抗体，可检测到变体之间在核衣壳（NP）蛋白上存在显著差异，在血凝素神经氨酸酶（HN）蛋白和磷蛋白（P）上也有细微变化。还发现病毒变体在感染脑不同区域的偏好性上存在差异。RK变体大量感染尾状核，而SK变体则不然。本研究表明，不同的传代方式可影响病毒粒子成分的特性和疾病模式。
