Kristensson K, Orvell C, Malm G, Norrby E
J Neuropathol Exp Neurol. 1984 Mar;43(2):131-40. doi: 10.1097/00005072-198403000-00003.
Newborn mice and hamsters were inoculated intracerebrally with mumps virus strains of high and low neurovirulence, Kilham and RW, respectively and with an egg-adapted patient isolate. The presence of viral antigen in brain tissue was analyzed with the immunofluorescence technique employing monoclonal antibodies against nucleoprotein (NP), polymerase (P), matrix (M), hemagglutinin-neuraminidase (HN) and fusion (F) mumps virus components. As expected, hamsters developed a fatal encephalitis eight to nine days after infection with the Kilham strain and synthesis of all five structural viral antigens was identified. In contrast, mice infected with any of the virus strains did not develop signs of disease, but in brain material collected on days nine and 12 after infection viral antigen was present in many neurons. However, only NP and P antigens were demonstrable and no infectious virus was present. The antibody response in mice developed later than in hamsters. Neurons in the mouse brain may exert a host cell restriction on the virus maturation, and mice offer a suitable host for the establishment of defective, persistent mumps virus infections.
新生小鼠和仓鼠分别脑内接种高神经毒力的腮腺炎病毒株Kilham和低神经毒力的腮腺炎病毒株RW，以及一株鸡胚适应的患者分离株。采用针对核蛋白（NP）、聚合酶（P）、基质（M）、血凝素 - 神经氨酸酶（HN）和融合（F）腮腺炎病毒成分的单克隆抗体，通过免疫荧光技术分析脑组织中病毒抗原的存在情况。正如预期的那样，仓鼠在感染Kilham株后八至九天发生致命性脑炎，并且鉴定出所有五种病毒结构抗原的合成。相比之下，感染任何一种病毒株的小鼠均未出现疾病迹象，但在感染后第9天和第12天收集的脑材料中，许多神经元中存在病毒抗原。然而，仅可证实NP和P抗原，且不存在感染性病毒。小鼠中的抗体反应比仓鼠出现得晚。小鼠脑中的神经元可能对病毒成熟发挥宿主细胞限制作用，并且小鼠为建立缺陷性、持续性腮腺炎病毒感染提供了合适的宿主。
