Wolinsky J S, Waxham M N, Server A C
J Virol. 1985 Mar;53(3):727-34. doi: 10.1128/JVI.53.3.727-734.1985.
Newborn Syrian hamsters were challanged with an intracerebral inoculum containing 128 50% lethal doses of the Kilham strain of mumps virus and treated 24 h later with a single intraperitoneal injection of mouse monoclonal antibody. Monoclonal antibodies reactive with epitopes on the fusion glycoprotein of mumps virus could not inhibit hemagglutination or neutralize infectivity in vitro and failed to provide biologically important protection against the in vivo infection. In contrast, monoclonal antibodies reactive with epitopes on the hemagglutinin-neuraminidase glycoprotein of mumps virus inhibited hemagglutination and neutralized infectivity in vitro and protected infected animals from the otherwise lethal central nervous system virus infection. Similar protection was provided by both purified immunoglobulin and F(ab')2 fragments. Immuno-cytochemical and virological studies showed diminished virus antigen and virus titers in the brains of successfully treated animals. It appears that a topographically restricted region of the hemagglutinin-neuraminidase molecule of the Kilham strain of mumps virus is of critical importance for immune recognition by the infected host.
将新生叙利亚仓鼠用含有128个50%致死剂量的腮腺炎病毒基哈姆株的脑内接种物进行攻击，并在24小时后用单次腹腔注射小鼠单克隆抗体进行治疗。与腮腺炎病毒融合糖蛋白表位反应的单克隆抗体在体外不能抑制血凝或中和感染性，并且不能为体内感染提供具有生物学重要性的保护。相比之下，与腮腺炎病毒血凝素神经氨酸酶糖蛋白表位反应的单克隆抗体在体外抑制血凝并中和感染性，并保护感染动物免受原本致命的中枢神经系统病毒感染。纯化的免疫球蛋白和F(ab')2片段都提供了类似的保护。免疫细胞化学和病毒学研究表明，成功治疗的动物大脑中的病毒抗原和病毒滴度降低。看来，腮腺炎病毒基哈姆株的血凝素神经氨酸酶分子的一个拓扑学上受限的区域对于感染宿主的免疫识别至关重要。
