用于蛋白质和核酸放射性化学标记的丰富培养基。
Rich culture medium for the radiochemical labeling of proteins and nucleic acids.
作者信息
Oeschger M P
出版信息
J Bacteriol. 1978 Jun;134(3):913-9. doi: 10.1128/jb.134.3.913-919.1978.
Abstract
Yeast extract was treated with tyrosine decarboxylase and used to prepare a rich, complex medium virtually free of tyrosine. The medium supported maximal growth rates for Escherichia coli prototrophs, as well as for defined and undefined auxotrophs. It has made possible the efficient radiochemical labeling of cells growing optimally in complex medium and the characterization of mutants with undefined requirements. Similarly prepared media may be useful for the study of fastidious organisms and organisms for which no defined medium has been described.
摘要
酵母提取物用酪氨酸脱羧酶处理后，用于制备一种几乎不含酪氨酸的丰富复杂培养基。该培养基支持大肠杆菌原养型以及特定和非特定营养缺陷型的最大生长速率。它使得在复杂培养基中最佳生长的细胞能够进行高效的放射化学标记，并对具有未明确需求的突变体进行表征。类似制备的培养基可能对研究苛求菌以及尚未描述特定培养基的生物体有用。
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本文引用的文献
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A simple scintillation counting technique for assaying C1402 in a Warburg flask.一种用于在瓦氏呼吸计中测定C1402的简单闪烁计数技术。
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