大肠杆菌K-12中serC基因（磷酸羟基丙酮酸转氨酶基因）与serS基因（丝氨酰转运核糖核酸合成酶基因）的紧密连锁。
Close linkage of the genes serC (for phosphohydroxy pyruvate transaminase) and serS (for seryl-transfer ribonucleic acid synthetase) in Escherichia coli K-12.
作者信息
Clarke S J, Low B, Konigsberg W H
出版信息
J Bacteriol. 1973 Mar;113(3):1091-5. doi: 10.1128/jb.113.3.1091-1095.1973.
Abstract
Escherichia coli strain K28, isolated after nitrosoguanidine mutagenesis, was found to be auxotrophic for serine. It was also temperature sensitive for growth as a result of producing an altered seryl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.11, l-serine: tRNA ligase [AMP]). The auxotrophy was caused by a mutation in the structural gene for phosphohydroxy-pyruvate transaminase (serC), which was distinct from, but closely linked to, the structural gene for seryl-tRNA synthetase (serS). We conclude that the relevant genes are in the order gal-serS-serC-aroA.
摘要
在经亚硝基胍诱变后分离得到的大肠杆菌K28菌株，被发现对丝氨酸营养缺陷。由于产生了一种改变的丝氨酰 - 转移核糖核酸（tRNA）合成酶（EC 6.1.1.11，L - 丝氨酸：tRNA连接酶[AMP]），它对生长也是温度敏感的。这种营养缺陷是由磷酸羟基丙酮酸转氨酶（serC）的结构基因突变引起的，该基因与丝氨酰 - tRNA合成酶（serS）的结构基因不同，但紧密连锁。我们得出结论，相关基因的顺序为gal - serS - serC - aroA。
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1
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J Biol Chem. 1951 Nov;193(1):265-75.
2
THE PATHWAY AND CONTROL OF SERINE BIOSYNTHESIS IN ESCHERICHIA COLI.大肠杆菌中丝氨酸生物合成的途径与调控
J Biol Chem. 1963 Dec;238:3934-44.
3
Genetic variation in the sex factor of Escherichia coli.大肠杆菌性别因子的遗传变异。
J Bacteriol. 1960 Mar;79(3):321-30. doi: 10.1128/jb.79.3.321-330.1960.
4
Hybridization between Escherichia coli and Shigella.大肠杆菌与志贺氏菌之间的杂交。
J Bacteriol. 1957 Oct;74(4):461-76. doi: 10.1128/jb.74.4.461-476.1957.
5
Isoleucine auxotrophy as a consequence of a mutationally altered isoleucyl-transfer ribonucleic acid synthetase.由于异亮氨酰 - 转移核糖核酸合成酶发生突变而导致的异亮氨酸营养缺陷。
J Bacteriol. 1971 Feb;105(2):527-37. doi: 10.1128/jb.105.2.527-537.1971.
6
A sensitive micro assay for inorganic pyrophosphatase.
Anal Biochem. 1970 Mar;34:66-70. doi: 10.1016/0003-2697(70)90086-2.
7
Roles of amino acid activating enzymes in cellular physiology.氨基酸活化酶在细胞生理学中的作用。
Bacteriol Rev. 1966 Dec;30(4):701-19. doi: 10.1128/br.30.4.701-719.1966.
8
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9
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