Jang Jiyi, Park Jiyeon, Hwang Chung Yeon, Choi Jinhee, Shin Jingyeong, Kim Young Mo, Cho Kyung Hwa, Kim Jung-Hyun, Lee Yung Mi, Lee Bang Yong
Korea Polar Research Institute, 26, Songdomirae-ro, Yeonsu-gu, Incheon 21990, South Korea; Ulsan National Institute of Science and Technology, 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan 44919, South Korea.
Korea Polar Research Institute, 26, Songdomirae-ro, Yeonsu-gu, Incheon 21990, South Korea.
Sci Total Environ. 2022 May 20;822:153360. doi: 10.1016/j.scitotenv.2022.153360. Epub 2022 Jan 24.
This study investigated the abundance and diversity of antibiotic resistance genes (ARGs) and the composition of bacterial communities along a transect covering the western Pacific Ocean (36°N) to the Southern Ocean (74°S) using the Korean icebreaker R/V Araon (total cruise distance: 14,942 km). The relative abundances of ARGs and bacteria were assessed with quantitative PCR and next generation sequencing, respectively. The absolute abundance of ARGs was 3.0 × 10 ± 1.6 × 10 copies/mL in the western Pacific Ocean, with the highest value (7.8 × 10 copies/mL) recorded at a station in the Tasman Sea (37°S). The absolute abundance of ARGs in the Southern Ocean was 1.8-fold lower than that in the western Pacific Ocean, and slightly increased (0.7-fold) toward Terra Nova Bay in Antarctica, possibly resulting from natural terrestrial sources or human activity. β-Lactam and tetracycline resistance genes were dominant in all samples (88-99%), indicating that they are likely the key ARGs in the ocean. Correlation and network analysis showed that Bdellovibrionota, Bacteroidetes, Cyanobacteria, Margulisbacteria, and Proteobacteria were positively correlated with ARGs, suggesting that these bacteria are the most likely ARG carriers. This study highlights the latitudinal profile of ARG distribution in the open ocean system and provides insights that will help in monitoring emerging pollutants on a global scale.
本研究利用韩国破冰船“R/V阿拉翁号”（总巡航距离：14,942千米），调查了从西太平洋（北纬36°）到南大洋（南纬74°）的一条断面沿线抗生素抗性基因（ARGs）的丰度和多样性以及细菌群落的组成。分别使用定量PCR和下一代测序技术评估了ARGs和细菌的相对丰度。西太平洋中ARGs的绝对丰度为3.0×10±1.6×10拷贝/毫升，在塔斯曼海（南纬37°）的一个站点记录到最高值（7.8×10拷贝/毫升）。南大洋中ARGs的绝对丰度比西太平洋低1.8倍，并且朝着南极洲的特拉诺瓦湾略有增加（0.7倍），这可能是自然陆地来源或人类活动导致的。β-内酰胺和四环素抗性基因在所有样本中占主导地位（88 - 99%），表明它们可能是海洋中的关键ARGs。相关性和网络分析表明，蛭弧菌门、拟杆菌门、蓝细菌门、马古利斯菌门和变形菌门与ARGs呈正相关，这表明这些细菌最有可能是ARGs的载体。本研究突出了公海系统中ARGs分布的纬度特征，并提供了有助于在全球范围内监测新出现污染物的见解。
