School of Life Sciences, Guangzhou University, Guangzhou 510006, China; State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory for Aquatic Economic Animals and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China.
School of Life Sciences, Guangzhou University, Guangzhou 510006, China.
Environ Pollut. 2022 Oct 15;311:119934. doi: 10.1016/j.envpol.2022.119934. Epub 2022 Aug 13.
Fish-related antibiotic resistance genes (ARGs) have attracted attention for their potentially harmful effects on food safety and human health through the food chain transfer. However, the potential factors affecting these ARGs have not been fully explored. In this study, ARGs and bacterial communities in the fish gut, mucosal skin, and gill filaments in fish were comprehensively evaluated in four different mariculture systems formed by hybrid grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂), Gracilaria bailinae, and Litopenaeus vannamei using different combinations. The results showed that 9 ARGs were detected in the gut and mucosal skin and 6 ARGs in the gill filaments. The detection rate of aphA1 was the highest, and the abundance was 1.91 × 10 - 6.30 × 10 copies per 16 S rRNA gene. Transposase gene (tnpA-04) was detected in all samples with the abundance of 3.57 × 10 - 3.59 × 10 copies per 16 S rRNA gene, and was strongly correlated with multiple ARGs (e.g., aphA1, tet(34), mphA-02). Proteobacteria, Deinococcus-Thermus, Firmicutes, and Bacteroidetes were the dominant phyla in the four mariculture systems, accounting for 65.1%-96.2% of the total bacterial community. Notably, the high relative abundance of Stenotrophomonas, a potential human pathogen, was elevated by 20.5% in the hybrid grouper gut in the monoculture system. In addition, variation partitioning analysis (VPA) showed that the difference in bacterial communities between mariculture systems was the main driving factor of ARGs distribution differences in hybrid groupers. This study provides a new comprehensive understanding of the characterization of fish-related ARGs contamination in different mariculture systems and facilitates the assessment of potential risks of ARGs and pathogen taxa to human health.
鱼类相关抗生素耐药基因（ARGs）通过食物链转移对食品安全和人类健康具有潜在危害作用，因此受到关注。然而，其潜在影响因素尚未得到充分探索。本研究综合评价了不同海水养殖系统中鱼类肠道、黏膜皮肤和鳃丝中的 ARGs 和细菌群落结构，这些养殖系统由杂交石斑鱼（♀ Epinephelus fuscoguttatus ×♂ E. lanceolatus）、条斑紫菜（Gracilaria bailinae）和凡纳滨对虾（Litopenaeus vannamei）组成。结果表明，在肠道和黏膜皮肤中检测到 9 种 ARGs，在鳃丝中检测到 6 种 ARGs。检测到的 aphA1 基因丰度最高，为 1.91×10 -6 -3.00×10 -6 拷贝数/16S rRNA 基因。tnpA-04 转座酶基因在所有样品中均有检出，丰度为 3.57×10 -3 -3.59×10 -3 拷贝数/16S rRNA 基因，与多种 ARGs（如 aphA1、tet(34)、mphA-02）呈显著正相关。四个海水养殖系统中的优势菌门为变形菌门（Proteobacteria）、脱氮球菌-栖热菌门（Deinococcus-Thermus）、厚壁菌门（Firmicutes）和拟杆菌门（Bacteroidetes），占总细菌群落的 65.1%-96.2%。值得注意的是，在单一养殖系统中，杂交石斑鱼肠道中潜在的人类病原菌 Stenotrophomonas 的相对丰度增加了 20.5%。此外，基于冗余分析（RDA）的方差分解分析（VPA）表明，养殖系统之间细菌群落的差异是导致杂交石斑鱼 ARGs 分布差异的主要驱动因素。本研究为不同海水养殖系统中鱼类相关 ARGs 污染特征提供了新的全面认识，有助于评估 ARGs 和病原体分类群对人类健康的潜在风险。
