MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200438, China.
Department of GDC Biology, Iowa State University, Ames, IA 50014, USA.
Biomed Res Int. 2022 Jan 18;2022:2229147. doi: 10.1155/2022/2229147. eCollection 2022.
Undeveloped ecosystems belong to rich source of microbial population, of which resources remain unearthed. A kind of polymeric compound system with high polyvinyl alcohol (PVA) content has been reported and named . Marker gene amplification showed that harbored little-explored microbial communities.
To address this issue, our study attempted to recover draft genomes and functional potential from microbial communities in using the metagenomic approach. . communities provided 97 novel bacterial genomes from 13 bacterial phyla, including bacteria candidate phylum. Two novel genus-level lineages were recovered from Planctomycetes and Chloroflexi. Based on the draft genomes, we expanded the number of taxa with potential productions of PKS and NRPS in phyla including Candidatus Dadabacteria, Chloroflexi, and Planctomycetes.
A rich diversity of PVA dehydrogenase genes from 4 phyla, involving Proteobacteria, Acidobacteria, Acitinobacteria, and Planctomycetes, were identified. The phylogenetic tree of PVA dehydrogenase showed the possibility of horizontal gene transfer between microbes.
Our study underscores the substantial microbial diversity and PVA degradation potential in the previously unexplored system.
未开发的生态系统属于微生物种群的丰富资源，其中的资源仍有待发掘。已经报道了一种高聚乙烯醇 (PVA) 含量的聚合化合物系统，并将其命名为 。标记基因扩增表明 蕴藏着尚未充分研究的微生物群落。
为了解决这个问题，我们的研究试图使用宏基因组学方法从 中的微生物群落中恢复草图基因组和功能潜力。 群落从 13 个细菌门中提供了 97 个新的细菌基因组，包括细菌候选门。从浮霉菌门和绿弯菌门中回收了两个新的属级谱系。基于草图基因组，我们扩展了具有 PKS 和 NRPS 潜在产物的分类单元数量，包括候选古菌门、绿弯菌门和浮霉菌门。
从 4 个门（包括变形菌门、酸杆菌门、放线菌门和浮霉菌门）中鉴定出了丰富多样的 PVA 脱氢酶基因。PVA 脱氢酶的系统发育树表明了微生物之间水平基因转移的可能性。
我们的研究强调了先前未探索的 系统中存在大量的微生物多样性和 PVA 降解潜力。
