Liu Zhigang, Yin Baishuang
College of Life Science, Anqing Normal University, Anqing, China.
Research Center of Aquatic Organism Conservation and Water Ecosystem Restoration in Anhui Province, Anqing Normal University, Anqing, China.
Front Vet Sci. 2022 Jan 13;8:778789. doi: 10.3389/fvets.2021.778789. eCollection 2021.
Hydatidosis/cystic echinococcosis (CE) caused by is a parasitic zoonotic disease worldwide, threatening animal health and production and public health safety. However, it is still unclear that whether infection can result in the alteration of gut microbiota in Tibetan sheep. Therefore, a study was designed to investigate the influences of infection on gut microbiota of Tibetan sheep. A total of 10 ovine small intestinal contents (five from healthy and five from infected) were obtained and subjected to high-throughput sequencing by MiSeq platform. A total of 2,395,641 sequences and 585 operational taxonomic units (OTUs) were identified. and were the most dominant phyla in all samples. Moreover, the proportions of and in the infected Tibetan sheep were significantly decreased, whereas , and had significantly increased. At the genus level, the R-7_group and NK4A214_group were the predominant bacterial genera in all the samples. Furthermore, the healthy Tibetan sheep exhibited higher abundances of group, , and , whereas , and levels were lower. In conclusion, our results conveyed an information that infection may cause an increase in pathogenic bacteria and a decrease in beneficial bacteria. Additionally, a significant dynamical change in gut microbiota could be associated with infection.
由细粒棘球绦虫引起的包虫病/囊型棘球蚴病（CE）是一种全球性的寄生虫人畜共患病，威胁着动物健康、生产以及公共卫生安全。然而，细粒棘球绦虫感染是否会导致藏羊肠道微生物群的改变仍不清楚。因此，设计了一项研究来调查细粒棘球绦虫感染对藏羊肠道微生物群的影响。总共获取了10份绵羊小肠内容物样本（5份来自健康绵羊，5份来自感染绵羊），并通过MiSeq平台进行高通量测序。共鉴定出2395641条序列和585个操作分类单元（OTU）。放线菌门和厚壁菌门是所有样本中最主要的门类。此外，感染细粒棘球绦虫的藏羊中放线菌门和厚壁菌门的比例显著降低，而变形菌门、拟杆菌门和梭杆菌门的比例显著增加。在属水平上，R-7_group和NK4A214_group是所有样本中占主导地位的细菌属。此外，健康藏羊中R-7_group、瘤胃球菌属和粪杆菌属的丰度较高，而埃希氏菌属、志贺氏菌属和链球菌属的水平较低。总之，我们的结果表明细粒棘球绦虫感染可能导致病原菌增加和有益菌减少。此外，肠道微生物群的显著动态变化可能与细粒棘球绦虫感染有关。
