Bao Jianling, Zheng Huajun, Wang Yuezhu, Zheng Xueting, He Li, Qi Wenjing, Wang Tian, Guo Baoping, Guo Gang, Zhang Zhaoxia, Zhang Wenbao, Li Jun, McManus Donald P
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China.
College of Public Health, Xinjiang Medical University, Urumqi, China.
Front Microbiol. 2018 Nov 29;9:2890. doi: 10.3389/fmicb.2018.02890. eCollection 2018.
Cystic echinococcosis (CE) is a chronic infectious disease caused by . To confirm whether the infection impacts on the gut microbiota, we established a mouse model of infection in this study whereby BALB/c mice were infected with micro-cysts of . After 4 months of infection, fecal samples were collected for high-throughput sequencing of the hypervariable regions of the 16S rRNA gene. Sequence analysis revealed a total of 13,353 operational taxonomic units (OTUs) with only 40.6% of the OTUs having genera reference information and 101 of the OTUs were significantly increased in infected mice. Bioinformatics analysis showed that the common core microbiota were not significantly changed at family level. However, two genera ( and ) were enriched in the infected mice ( < 0.05) at genus level. Functional analysis indicated that seven pathways were altered in the Infection Group compared with the Uninfected Group. Spearman correlation analysis showed strong correlations of IgG, IgG1 and IgG2a with nine major genera. cyst infection may change the gut microbiota which may be associated with metabolic pathways.
囊型包虫病（CE）是一种由……引起的慢性传染病。为了确定该感染是否会影响肠道微生物群，我们在本研究中建立了一种感染小鼠模型，即给BALB/c小鼠感染……的微囊。感染4个月后，收集粪便样本用于16S rRNA基因高变区的高通量测序。序列分析共鉴定出13353个可操作分类单元（OTU），其中只有40.6%的OTU有属参考信息，且101个OTU在感染小鼠中显著增加。生物信息学分析表明，在科水平上，常见核心微生物群没有显著变化。然而，在属水平上，两个属（……和……）在感染小鼠中富集（……<0.05）。功能分析表明，与未感染组相比，感染组有7条代谢途径发生改变。Spearman相关性分析显示IgG、IgG1和IgG2a与9个主要菌属有强相关性。……囊感染可能会改变肠道微生物群，这可能与代谢途径有关。
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