Brawner D L, Cutler J E
Infect Immun. 1986 Jan;51(1):337-43. doi: 10.1128/iai.51.1.337-343.1986.
The location and expression of two different cell surface antigens on germinating and nongerminating Candida albicans cells was examined by using transmission electron microscopy after labeling with monoclonal antibodies (H9 or C6) and immunocolloidal gold. Immunodeterminant expression of the two carbohydrate antigens was followed from early germination events through 20 h of development. The determinant detected by H9 antibody, which was initially lost from the mother cell surface and preferentially expressed only on hyphae during the first 4 h of germination, reappeared on the mother cell by 20 h, whereas the antigen detected by antibody C6 was continually expressed on mother cells and germ tubes throughout germination.
在用单克隆抗体（H9或C6）和免疫胶体金标记后，通过透射电子显微镜检查了两种不同细胞表面抗原在发芽和未发芽的白色念珠菌细胞上的定位和表达。从早期发芽事件到发育20小时，追踪了这两种碳水化合物抗原的免疫决定簇表达情况。H9抗体检测到的决定簇最初从母细胞表面消失，在发芽的最初4小时内仅优先在菌丝上表达，到20小时时又重新出现在母细胞上，而抗体C6检测到的抗原在整个发芽过程中持续在母细胞和芽管上表达。
