Brawner D L, Cutler J E
Department of Microbiology, Montana State University, Bozeman 59717.
Microb Pathog. 1987 Apr;2(4):249-57. doi: 10.1016/0882-4010(87)90123-9.
Intracellular and cell surface expression of two antigenic determinants of Candida albicans was studied by reacting ultrathin sections of the fungus with monoclonal antibodies and locating the sites of antibody-antigen reactions by use of colloidal gold-coupled secondary antibody and electron microscopic techniques. Comparisons were made between fungal cells allowed to develop in vitro and cells (in vivo) recovered from infected mice. Differences between in vitro antigen expression and expression in vivo were particularly noted during early germination events. Antigen expression in vivo appears to be concentrated in deeper cell wall layers and beneath the plasmalemma especially on germ tubes. Cells which develop in vivo and in vitro have antigen associated with an outer flocculent layer and on the innermost cell wall layer of mother cells and hyphae. A middle electron transparent cell wall layer is usually devoid of antigen. Comparison of antigen expression during in vivo growth in early (intraperitoneal) infection versus late disseminated (kidney) disease suggests that both of the antigens are expressed in greater quantity on the surface of germ tubes than on mother cells in kidney tissue as compared to intraperitoneally-grown cells. Results also suggest that at least one of the antigens, which is a substituted mannan, can be found on a lamello-tubular intracellular structure.
通过将白色念珠菌的超薄切片与单克隆抗体反应，并使用胶体金偶联二抗和电子显微镜技术定位抗体 - 抗原反应位点，研究了白色念珠菌两种抗原决定簇的细胞内和细胞表面表达。对体外培养的真菌细胞与从感染小鼠体内分离的细胞（体内）进行了比较。在早期萌发过程中，特别注意到体外抗原表达与体内表达之间的差异。体内抗原表达似乎集中在更深的细胞壁层和质膜下方，尤其是在芽管上。在体内和体外生长的细胞在母细胞和菌丝的最外层絮状层以及最内层细胞壁层上都有抗原。中间的电子透明细胞壁层通常没有抗原。比较早期（腹腔内）感染与晚期播散性（肾脏）疾病期间体内生长过程中的抗原表达，结果表明，与腹腔内生长的细胞相比，在肾脏组织中，两种抗原在芽管表面的表达量均高于母细胞。结果还表明，至少一种抗原是取代甘露聚糖，可在层状管状细胞内结构上发现。
