Department of Chemistry and Biochemistry, Hampton University, 200 William R. Harvey Way, Hampton, VA 23668, USA.
UCB Pharma, Bedford, Massachusetts, USA.
Acta Crystallogr F Struct Biol Commun. 2022 Feb 1;78(Pt 2):52-58. doi: 10.1107/S2053230X22000218. Epub 2022 Jan 27.
Burkholderia phymatum is an important symbiotic nitrogen-fixing betaproteobacterium. B. phymatum is beneficial, unlike other Burkholderia species, which cause disease or are potential bioagents. Structural genomics studies at the SSGCID include characterization of the structures of short-chain dehydrogenases/reductases (SDRs) from multiple Burkholderia species. The crystal structure of a short-chain dehydrogenase from B. phymatum (BpSDR) was determined in space group C222 at a resolution of 1.80 Å. BpSDR shares less than 38% sequence identity with any known structure. The monomer is a prototypical SDR with a well conserved cofactor-binding domain despite its low sequence identity. The substrate-binding cavity is unique and offers insights into possible functions and likely inhibitors of the enzymatic functions of BpSDR.
鲍氏不动杆菌是一种重要的共生固氮β变形菌。不同于其他引起疾病或具有潜在生物制剂风险的伯克霍尔德菌属物种，鲍氏不动杆菌是有益的。在 SSGCID 进行的结构基因组学研究包括对多种伯克霍尔德菌属物种的短链脱氢酶/还原酶（SDR）结构进行了特征描述。从鲍氏不动杆菌（BpSDR）中确定了短链脱氢酶的晶体结构，空间群为 C222，分辨率为 1.80 Å。BpSDR 与任何已知结构的序列同一性小于 38%。单体是一个典型的 SDR，尽管其序列同一性较低，但辅酶结合结构域高度保守。底物结合腔是独特的，为 BpSDR 的酶功能的可能功能和潜在抑制剂提供了深入了解。
